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Literature summary for 2.1.1.13 extracted from

  • Prasannan, P.; Suliman, H.S.; Robertus, J.D.
    Kinetic analysis of site-directed mutants of methionine synthase from Candida albicans (2009), Biochem. Biophys. Res. Commun., 382, 730-734.
    View publication on PubMed
Search for more literature for 2.1.1.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a GST fusion protein in Saccharomyces cerevisiae strain SDYalpha Candida albicans

Protein Variants

Protein Variants Comment Organism
D504A the mutant exhibits 4% of wild type activity Candida albicans
D504N the mutant exhibits 11% of wild type activity Candida albicans
D504S the mutant exhibits 7% of wild type activity Candida albicans
D614A the mutant exhibits less than 2% of wild type activity Candida albicans
D614N the mutant exhibits less than 2% of wild type activity Candida albicans
D614S the mutant exhibits 11% of wild type activity Candida albicans
E620Q the mutation has no effect on enzyme activity Candida albicans
E620S the mutation has no effect on enzyme activity Candida albicans
H128N the mutant is 5fold less active compared to the wild type GST fusion protein Candida albicans
R530A the mutant exhibits 33% of wild type activity Candida albicans
S448A the mutant exhibits 67% of wild type activity Candida albicans
W576F the mutant is 3fold less active compared to the wild type GST fusion protein Candida albicans
W576Y the mutant is 8fold less active compared to the wild type GST fusion protein Candida albicans
Y527F the mutant exhibits 67% of wild type activity Candida albicans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 L-homocysteine in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-HCl (pH 7.2), 0.1 mM MgSO4, and 10 mM dithiothreitol, at 37°C Candida albicans
0.165
-
 N5-methyltetrahydropteroylmonoglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-HCl (pH 7.2), 0.1 mM MgSO4, and 10 mM dithiothreitol, at 37°C Candida albicans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
 SDS-PAGE Candida albicans
112000
-
 GST fusion protein, SDS-PAGE Candida albicans

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione agarose column chromatography Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Candida albicans tetrahydropteroylmonoglutamate + L-methionine
-
 ?

Synonyms

Synonyms Comment Organism
Met6p
-
 Candida albicans