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Literature summary for 2.1.1.13 extracted from

  • Wolthers, K.R.; Toogood, H.S.; Jowitt, T.A.; Marshall, K.R.; Leys, D.; Scrutton, N.S.
    Crystal structure and solution characterization of the activation domain of human methionine synthase (2007), FEBS J., 274, 738-750.
    View publication on PubMed
Search for more literature for 2.1.1.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method in reservoir solution of 0.1 M Tris/HCl pH 7.5 containing 0.1-0.4 M sodium acetate, 10-12% poly(ethylene glycol) 8000 and 10-12% poly(ethyleneglycol) 1000 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D963E /K1071N reduced activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36200
-
 sedimentation in high salt buffer Homo sapiens
40000
-
 2 * 40000, SDS-PAGE Homo sapiens
43100
-
 1 * 43100, light scattering Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin HisTrap column chromatography, HiPrep Q Sepharose column chromatography, and Resource Q column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Homo sapiens tetrahydropteroylmonoglutamate + L-methionine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Homo sapiens
monomer 1 * 43100, light scattering Homo sapiens

Synonyms

Synonyms Comment Organism
5-methyltetrahydrofolate homocysteine methyltransferase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
vitamin B12 dependent Homo sapiens