Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thalictrum flavum subsp. glaucum |
Crystallization (Comment) | Organism |
---|---|
structures of apo-6OMT, the binary complex 6OMT/SAH and the two ternary complexes 6OMT/SAH/norlaudanosoline and 6OMT/SAH/sanguinarine, refined to a resolution of 2.6, 1.9, 1.6 and 1.8 A, respectively | Thalictrum flavum subsp. glaucum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: berberine up to 1 mM | Thalictrum flavum subsp. glaucum | |
sanguinarine | slow tight-binding inhibitor | Thalictrum flavum subsp. glaucum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
(R,S)-norlaudanosoline | pH 8.0, 30°C | Thalictrum flavum subsp. glaucum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
gel filtration, recombinant protein | Thalictrum flavum subsp. glaucum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thalictrum flavum subsp. glaucum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + (R,S)-norlaudanosoline | - |
Thalictrum flavum subsp. glaucum | S-adenosyl-L-homocysteine + (RS)-6-O-methylnorlaudanosoline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6OMT | - |
Thalictrum flavum subsp. glaucum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
(R,S)-norlaudanosoline | pH 8.0, 30°C | Thalictrum flavum subsp. glaucum |