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Literature summary for 2.1.1.128 extracted from

  • Rueffer, M.; Nagakura, N.; Zenk, M.H.
    Partial purification and properties of S-adenosylmethionine:(R),(S)-norlaudanosoline-6-O-methyltransferase from Argemone platyceras cell culture (1983), J. Med. Plant Res., 49, 131-137.
    View publication on PubMed
Search for more literature for 2.1.1.128

General Stability

General Stability Organism
complete loss of activity after freezing in 30% glycerol solution Argemone platyceras

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
 Argemone platyceras
Cu2+
-
 Argemone platyceras
Fe2+
-
 Argemone platyceras
Hg2+
-
 Argemone platyceras
iodobenzoate
-
 Argemone platyceras
Mn2+
-
 Argemone platyceras
Ni2+
-
 Argemone platyceras
PCMB
-
 Argemone platyceras
S-adenosyl-L-homocysteine
-
 Argemone platyceras
Sn2+
-
 Argemone platyceras
Zn2+
-
 Argemone platyceras

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 S-adenosylmethionine with (S)-norlaudanosoline as cosubstrate Argemone platyceras
0.2
-
 (R,S)-norlaudanosoline
-
 Argemone platyceras
0.3
-
 (R,S)-laudanosoline
-
 Argemone platyceras
1.1
-
 (R,S)-4'-O-methylnorlaudanosoline
-
 Argemone platyceras

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 gel filtration Argemone platyceras

Organism

Organism UniProt Comment Textmining
Argemone platyceras
-
-
-
Papaver somniferum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Argemone platyceras

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Papaver somniferum
-
cell culture
-
 Argemone platyceras
-

Storage Stability

Storage Stability Organism
4°C, 50% loss of activity after 4 weeks Argemone platyceras

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-Adenosyl-L-methionine + (R)-norlaudanosoline 92% of the activity with (S)-norlaudanosoline Argemone platyceras S-Adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline in the ratio 8:2 ?
S-Adenosyl-L-methionine + (R,S)-2,3-dihydroxy-9,10-dimethoxytetrahydroprotoberberine 5% of the activity with (S)-norlaudanosoline Argemone platyceras S-Adenosyl-L-homocysteine + ?
-
 ?
S-Adenosyl-L-methionine + (R,S)-4'-O-methylnorlaudanosoline 34% of the activity with (S)-norlaudanosoline Argemone platyceras S-Adenosyl-L-homocysteine + norprotosinomenine
-
 ?
S-Adenosyl-L-methionine + (R,S)-5'-O-methylnorlaudanosoline 81% of the activity with (S)-norlaudanosoline Argemone platyceras S-Adenosyl-L-homocysteine + 6-O-methyllaudanosoline
-
 ?
S-adenosyl-L-methionine + (R,S)-norlaudanosoline
-
 Argemone platyceras S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline
-
 ?
S-adenosyl-L-methionine + (S)-norlaudanosoline
-
 Argemone platyceras S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline in the ratio 8:2 ?
S-Adenosyl-L-methionine + (S)-scoulerine 1% of the activity with (S)-norlaudanosoline Argemone platyceras S-Adenosyl-L-homocysteine + jatrorrhizine
-
 ?
S-Adenosyl-L-methionine + 2,3-dihydroxy-9,10-dimethoxyprotoberberine 7% of the activity with (S)-norlaudanosoline Argemone platyceras S-Adenosyl-L-homocysteine + tetrahydrojatrorrhizine
-
 ?
S-Adenosyl-L-methionine + laudanosoline 79% of the activity with (S)-norlaudanosoline Argemone platyceras S-Adenosyl-L-homocysteine + tetrahydrocolumbamine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Argemone platyceras

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 half-life: 8 h Argemone platyceras

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Argemone platyceras