Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21, His-tagged protein | Pisum sativum |
Crystallization (Comment) | Organism |
---|---|
complexed with S-adenosyl-L-homocysteine, hanging drop method, 25°C, reservoir solution contains HEPES 100 mM, pH 6.8, sodium acetate 1.2-1.35 M, structure analysis | Pisum sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | - |
Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pisum sativum | histone methylation has significant effects on heterochromatin formation and transcriptional regulation | ? | - |
? | |
S-adenosyl-L-methionine + histone L-lysine | Pisum sativum | - |
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from E. coli | Pisum sativum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | catalytic mechanism | Pisum sativum | |
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | substrate and inhibitor binding site | Pisum sativum | |
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | amino acid sequence comparison | Pisum sativum | |
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | enzyme is encoded on genes containing the SET-domain sequence, substrate specificity is connected to SET sequence | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | histone methylation has significant effects on heterochromatin formation and transcriptional regulation | Pisum sativum | ? | - |
? | |
S-adenosyl-L-methionine + histone L-lysine | - |
Pisum sativum | S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pisum sativum |