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Literature summary for 2.1.1.122 extracted from
- Isolation and characterization of S-adenosyl-L-methionine:tetrahydroberberibe-cis-N-methyltransferase from suspension cultures of Sanguinaria canadensis L. (1994), Plant Physiol., 105, 395-403.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Berberine | - |
Sanguinaria canadensis |  |
| Ca2+ | in Hepes buffer, not inhibitory in sodium phosphate buffer | Sanguinaria canadensis | |
| Co2+ | in sodium phosphate buffer and in Hepes buffer | Sanguinaria canadensis | |
| Mg2+ | slight increase in activity with increasing concentrations of Mg2+ in sodium phosphate buffer, inhibitory in Hepes buffer | Sanguinaria canadensis | |
| NEM | - |
Sanguinaria canadensis | |
| S-adenosyl-L-homocysteine | - |
Sanguinaria canadensis | |
| Zn2+ | in sodium phosphate buffer and in Hepes buffer | Sanguinaria canadensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
S-adenosyl-L-methionine | - |
Sanguinaria canadensis | |
| 0.0021 | - |
(S)-7,8,13,14-tetrahydroprotoberberine | - |
Sanguinaria canadensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | slight increase in activity with increasing concentrations of Mg2+ in sodium phosphate buffer, inhibitory in Hepes buffer | Sanguinaria canadensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
2 * 39000, SDS-PAGE | Sanguinaria canadensis |
| 70000 | - |
gel filtration | Sanguinaria canadensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sanguinaria canadensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sanguinaria canadensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Sanguinaria canadensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Sanguinaria canadensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-Adenosyl-L-methionine + (S)-7,8,13,14-tetrahydroprotoberberine | - |
Sanguinaria canadensis | S-Adenosyl-L-homocysteine + cis-N-methyl-(S)-7,8,13,14-tetrahydroprotoberberine | - |
? | |
| S-Adenosyl-L-methionine + tetrahydrojatrorrhizine | - |
Sanguinaria canadensis | ? | - |
? | |
| S-Adenosyl-L-methionine + tetrahydropalmatrubine | - |
Sanguinaria canadensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 39000, SDS-PAGE | Sanguinaria canadensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | - |
Sanguinaria canadensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 40 | 15°C: about 30% of maximal activity, 40°C: about 25% of maximal activity | Sanguinaria canadensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Sanguinaria canadensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | pH 6.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity | Sanguinaria canadensis |
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