BRENDA - Enzyme Database show
show all sequences of 2.1.1.118

S-Adenosyl-L-methionine:columbamine-O-methyl transferase, a compartmentalized enzyme in protoberberine biosynthesis

Rueffer, M.; Amann, M.; Zenk, M.H.; Plant Cell Rep. 3, 182-185 (1986)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
S-adenosyl-L-homocysteine
-
Berberis wilsoniae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0013
-
Columbamine
-
Berberis aggregata
0.0013
-
Columbamine
-
Berberis wilsoniae
0.0015
-
S-adenosyl-L-methionine
-
Berberis aggregata
0.0015
-
S-adenosyl-L-methionine
-
Berberis wilsoniae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
vesicular fraction
-
Berberis aggregata
-
-
vesicular fraction
-
Berberis wilsoniae
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
gel filtration
Berberis aggregata
52000
-
gel filtration
Berberis wilsoniae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Berberis aggregata
-
-
-
Berberis wilsoniae
-
var. subcaulialate
-
Purification (Commentary)
Commentary
Organism
partial
Berberis aggregata
partial
Berberis wilsoniae
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Berberis aggregata
-
cell suspension culture
-
Berberis wilsoniae
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + columbamine
-
441450
Berberis wilsoniae
S-adenosyl-L-homocysteine + palmatine
-
-
-
?
S-adenosyl-L-methionine + columbamine
-
441450
Berberis aggregata
S-adenosyl-L-homocysteine + palmatine
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
-
-
Berberis aggregata
20
-
-
Berberis wilsoniae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
S-adenosyl-L-homocysteine
-
Berberis wilsoniae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0013
-
Columbamine
-
Berberis aggregata
0.0013
-
Columbamine
-
Berberis wilsoniae
0.0015
-
S-adenosyl-L-methionine
-
Berberis aggregata
0.0015
-
S-adenosyl-L-methionine
-
Berberis wilsoniae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
vesicular fraction
-
Berberis aggregata
-
-
vesicular fraction
-
Berberis wilsoniae
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
gel filtration
Berberis aggregata
52000
-
gel filtration
Berberis wilsoniae
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Berberis aggregata
partial
Berberis wilsoniae
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Berberis aggregata
-
cell suspension culture
-
Berberis wilsoniae
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + columbamine
-
441450
Berberis wilsoniae
S-adenosyl-L-homocysteine + palmatine
-
-
-
?
S-adenosyl-L-methionine + columbamine
-
441450
Berberis aggregata
S-adenosyl-L-homocysteine + palmatine
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
-
-
Berberis aggregata
20
-
-
Berberis wilsoniae
Other publictions for EC 2.1.1.118
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
441451
Morishige
Molecular cloning of columbami ...
Coptis japonica
Eur. J. Biochem.
269
5659-5667
2002
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1
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-
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-
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3
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1
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1
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1
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-
-
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-
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-
-
-
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-
441450
Rueffer
-
S-Adenosyl-L-methionine:columb ...
Berberis aggregata, Berberis wilsoniae
Plant Cell Rep.
3
182-185
1986
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1
4
2
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2
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2
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2
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2
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2
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2
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1
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4
2
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2
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2
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2
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2
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2
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