BRENDA - Enzyme Database show
show all sequences of 2.1.1.117

S-Adenosyl-L-methionine:(S)-scoulerine 9-O-methyltransferase, a highly stereo- and regio-specific enzyme in tetrahydroprotoberberine biosynthesis

Muemmler, S.; Rueffer, M.; Nagakura, N.; Zenk, M.H.; Plant Cell Rep. 4, 36-39 (1985)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
5 mM, 97% inhibition
Berberis wilsoniae
S-adenosylhomocysteine
-
Berberis wilsoniae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.042
-
S-adenosyl-L-methionine
-
Berberis wilsoniae
1.6
-
scoulerine
-
Berberis wilsoniae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
63000
-
-
Berberis wilsoniae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-Adenosyl-L-methionine + (S)-scoulerine
Berberis wilsoniae
central role in orotoberberine biosynthesis
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Berberis wilsoniae
-
var. subcauliata
-
Purification (Commentary)
Commentary
Organism
-
Berberis wilsoniae
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Berberis wilsoniae
-
cell suspension culture
-
Berberis wilsoniae
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Berberis wilsoniae
Storage Stability
Storage Stability
Organism
-20°C, 20% glycerol, 50% loss of activity after 7 days
Berberis wilsoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-Adenosyl-L-methionine + (R,S)-2,3,9,10-tetrahydroxytetrahydroprotoberberine
methylated at 50% of the rate of (S)-scoulerine
15196
Berberis wilsoniae
?
-
-
-
-
S-Adenosyl-L-methionine + (S)-3,9-dihydroxy-2,10-dimethoxytetrahydroprotoberberine
methylated at 4% of the rate of (S)-scoulerine
15196
Berberis wilsoniae
?
-
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
-
15196
Berberis wilsoniae
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
15196
Berberis wilsoniae
-
S-Adenosyl-L-methionine + (S)-scoulerine
central role in orotoberberine biosynthesis
15196
Berberis wilsoniae
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Berberis wilsoniae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.9
-
-
Berberis wilsoniae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
5 mM, 97% inhibition
Berberis wilsoniae
S-adenosylhomocysteine
-
Berberis wilsoniae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.042
-
S-adenosyl-L-methionine
-
Berberis wilsoniae
1.6
-
scoulerine
-
Berberis wilsoniae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
63000
-
-
Berberis wilsoniae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-Adenosyl-L-methionine + (S)-scoulerine
Berberis wilsoniae
central role in orotoberberine biosynthesis
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Berberis wilsoniae
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Berberis wilsoniae
-
cell suspension culture
-
Berberis wilsoniae
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Berberis wilsoniae
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 20% glycerol, 50% loss of activity after 7 days
Berberis wilsoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-Adenosyl-L-methionine + (R,S)-2,3,9,10-tetrahydroxytetrahydroprotoberberine
methylated at 50% of the rate of (S)-scoulerine
15196
Berberis wilsoniae
?
-
-
-
-
S-Adenosyl-L-methionine + (S)-3,9-dihydroxy-2,10-dimethoxytetrahydroprotoberberine
methylated at 4% of the rate of (S)-scoulerine
15196
Berberis wilsoniae
?
-
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
-
15196
Berberis wilsoniae
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
15196
Berberis wilsoniae
-
S-Adenosyl-L-methionine + (S)-scoulerine
central role in orotoberberine biosynthesis
15196
Berberis wilsoniae
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Berberis wilsoniae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.9
-
-
Berberis wilsoniae
Other publictions for EC 2.1.1.117
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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727532
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2
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733669
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Homology modeling and molecula ...
Coptis chinensis
Chin. J. Chem.
30
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2012
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720655
Takemura
Metabolic diversification of b ...
Coptis japonica
Plant Cell Physiol.
51
949-959
2010
-
-
1
-
-
-
-
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5
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1
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1
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1
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1
1
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658313
Dubouzet
Transient RNA silencing of sco ...
Coptis japonica
Biosci. Biotechnol. Biochem.
69
63-70
2005
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1
-
-
-
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1
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3
-
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15194
Takeshita
Molecular cloning and characte ...
Coptis japonica
Plant Cell Physiol.
36
29-36
1995
-
-
1
-
-
-
-
-
-
-
1
1
-
1
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-
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-
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-
2
1
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1
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1
1
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2
1
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-
15192
Fujiwara
-
Expression of (S)-scoulerine 9 ...
Coptis japonica
Phytochemistry
34
949-954
1993
-
-
-
-
-
-
-
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-
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1
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1
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4
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2
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1
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4
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2
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15193
Sato
-
S-Adenosyl-L-methionine:scoule ...
Coptis japonica
Phytochemistry
32
659-664
1993
-
-
-
-
-
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7
2
-
-
2
-
-
1
-
-
1
-
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4
1
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1
1
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7
-
2
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2
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1
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4
1
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1
1
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-
15195
Galneder
Alternative final steps in ber ...
Berberis stolonifera, Coptis japonica
Plant Cell Rep.
7
1-4
1988
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-
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2
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2
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4
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4
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-
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-
15196
Muemmler
S-Adenosyl-L-methionine:(S)-sc ...
Berberis wilsoniae
Plant Cell Rep.
4
36-39
1985
-
-
-
-
-
-
2
2
-
-
1
1
-
2
-
-
1
-
-
2
1
1
4
-
1
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-
-
1
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2
-
2
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1
1
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1
-
2
1
1
4
-
1
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1
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