BRENDA - Enzyme Database show
show all sequences of 2.1.1.117

Alternative final steps in berberine biosynthesis in Coptis japonica cell cultures

Galneder, E.; Rueffer, M.; Wanner, G.; Tabata, M.; Zenk, M.H.; Plant Cell Rep. 7, 1-4 (1988)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-Adenosyl-L-methionine + (S)-scoulerine
Berberis stolonifera
-
?
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
Coptis japonica
the enzyme is responsible for a late step in the biosynthesis of berberine
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Berberis stolonifera
-
-
-
Coptis japonica
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-Adenosyl-L-methionine + (S)-scoulerine
-
15195
Berberis stolonifera
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
-
15195
Coptis japonica
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
-
15195
Berberis stolonifera
?
-
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
the enzyme is responsible for a late step in the biosynthesis of berberine
15195
Coptis japonica
?
-
-
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-Adenosyl-L-methionine + (S)-scoulerine
Berberis stolonifera
-
?
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
Coptis japonica
the enzyme is responsible for a late step in the biosynthesis of berberine
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-Adenosyl-L-methionine + (S)-scoulerine
-
15195
Berberis stolonifera
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
-
15195
Coptis japonica
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
-
15195
Berberis stolonifera
?
-
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
the enzyme is responsible for a late step in the biosynthesis of berberine
15195
Coptis japonica
?
-
-
-
-
Other publictions for EC 2.1.1.117
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743234
Galanie
Optimization of yeast-based p ...
Thalictrum flavum subsp. glaucum
Microb. Cell Fact.
14
144
2015
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727532
Dang
Cloning and characterization o ...
Papaver somniferum
FEBS Lett.
588
198-204
2014
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733669
Zhu
-
Homology modeling and molecula ...
Coptis chinensis
Chin. J. Chem.
30
2533-2538
2012
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
720655
Takemura
Metabolic diversification of b ...
Coptis japonica
Plant Cell Physiol.
51
949-959
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
658313
Dubouzet
Transient RNA silencing of sco ...
Coptis japonica
Biosci. Biotechnol. Biochem.
69
63-70
2005
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15194
Takeshita
Molecular cloning and characte ...
Coptis japonica
Plant Cell Physiol.
36
29-36
1995
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15192
Fujiwara
-
Expression of (S)-scoulerine 9 ...
Coptis japonica
Phytochemistry
34
949-954
1993
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15193
Sato
-
S-Adenosyl-L-methionine:scoule ...
Coptis japonica
Phytochemistry
32
659-664
1993
-
-
-
-
-
-
7
2
-
-
2
-
-
1
-
-
1
-
-
-
-
-
4
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
-
2
-
-
-
-
1
-
-
-
-
4
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
15195
Galneder
Alternative final steps in ber ...
Berberis stolonifera, Coptis japonica
Plant Cell Rep.
7
1-4
1988
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15196
Muemmler
S-Adenosyl-L-methionine:(S)-sc ...
Berberis wilsoniae
Plant Cell Rep.
4
36-39
1985
-
-
-
-
-
-
2
2
-
-
1
1
-
2
-
-
1
-
-
2
1
1
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
1
1
-
-
-
1
-
2
1
1
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-