BRENDA - Enzyme Database show
show all sequences of 2.1.1.113

DNA-(N4-cytosine)-methyltransferase from Bacillus amyloliquefaciens: kinetic and substrate-binding properties

Malygin, E.G.; Ovechkina, L.G.; Zinoviev, V.V.; Lindstrem, U.M.; Reich, N.O.; Mol. Biol. (Mosk.) 35, 35-44 (2001)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00035
-
5'-CAGTTTAGGATCCATTTCAC-3'/3'-GTCAAATCCTAGGTAAAAGAG-5'
-
Bacillus amyloliquefaciens
0.0016
-
S-adenosyl-L-methionine
-
Bacillus amyloliquefaciens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus amyloliquefaciens
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + [DNA]-cytosine
20-mer duplex 5'-CAGTTTAGGATCCATTTCAC-3'/3'-GTCAAATCCTAGGTAAAAGAG-5' containing a palindromic recognition site GGATCC
485137
Bacillus amyloliquefaciens
S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
485137
Bacillus amyloliquefaciens
?
Subunits
Subunits
Commentary
Organism
More
enzyme in a free state exists as a dimer. Introduction of substoichiometric amounts of DNA into the reaction mixture results in pronounced multimerization of the enzyme. However, addition of SAM in saturating concentration at an excess of the oligonucleotide duplex over BamHI Mtase converts most of the enzyme into a monomeric state
Bacillus amyloliquefaciens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Bacillus amyloliquefaciens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00035
-
5'-CAGTTTAGGATCCATTTCAC-3'/3'-GTCAAATCCTAGGTAAAAGAG-5'
-
Bacillus amyloliquefaciens
0.0016
-
S-adenosyl-L-methionine
-
Bacillus amyloliquefaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + [DNA]-cytosine
20-mer duplex 5'-CAGTTTAGGATCCATTTCAC-3'/3'-GTCAAATCCTAGGTAAAAGAG-5' containing a palindromic recognition site GGATCC
485137
Bacillus amyloliquefaciens
S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
485137
Bacillus amyloliquefaciens
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
enzyme in a free state exists as a dimer. Introduction of substoichiometric amounts of DNA into the reaction mixture results in pronounced multimerization of the enzyme. However, addition of SAM in saturating concentration at an excess of the oligonucleotide duplex over BamHI Mtase converts most of the enzyme into a monomeric state
Bacillus amyloliquefaciens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Bacillus amyloliquefaciens
Other publictions for EC 2.1.1.113
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734997
Furuta
Methylome diversification thro ...
Helicobacter pylori
PLoS Genet.
10
e1004272
2014
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720604
Clark
Characterization of DNA methyl ...
Acetobacter aceti, Geobacillus stearothermophilus, Rhodobacter sphaeroides, uncultured bacterium
Nucleic Acids Res.
40
e29
2012
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734823
Aranda
Substrate promiscuity in DNA m ...
Proteus vulgaris
Org. Biomol. Chem.
10
5395-5400
2012
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735063
Chung
Methylation by a unique alpha- ...
Caldicellulosiruptor bescii, Caldicellulosiruptor bescii DSM 6725
PLoS ONE
7
e43844
2012
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1
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720306
Aranda
Theoretical study of the catal ...
Proteus vulgaris
J. Phys. Chem. B
114
8467-8473
2010
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1
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705556
Furmanek-Blaszk
M1.Mboll and M2.Mboll type IIS ...
Moraxella bovis, Moraxella cuniculi
Microbiology
155
1111-1121
2009
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2
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8
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8
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2
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694446
Carpenter
DNA base flipping by both memb ...
Pyrococcus sp. GI-H
Nucleic Acids Res.
36
5417-5425
2008
1
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1
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686788
Yu
A novel restriction-modificati ...
Xanthomonas campestris
FEMS Microbiol. Lett.
272
83-90
2007
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1
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660031
Zinoviev
Symmetry elements in DNA struc ...
Bacillus amyloliquefaciens
Nucleic Acids Res.
32
3930-3934
2004
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485128
Malygin
-
DNA-(cytosine-N4)- and (adenin ...
Bacillus amyloliquefaciens
J. Biol. Chem.
20
1-31
2003
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485130
Zinoviev
-
DNA-(N4-Cytosine)-methyltransf ...
Bacillus amyloliquefaciens
Mol. Biol. (Mosk.)
37
116-124
2003
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485131
Lindstrom
Functional analysis of BamHI D ...
Bacillus amyloliquefaciens
J. Mol. Biol.
325
711-720
2003
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725232
Grogan
Cytosine methylation by the Su ...
Sulfolobus acidocaldarius, Sulfolobus acidocaldarius DG185
J. Bacteriol.
185
4657-4661
2003
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4
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485136
Jeltsch
The cytosine N4-methyltransfer ...
Proteus vulgaris
Biol. Chem.
382
707-710
2001
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485137
Malygin
-
DNA-(N4-cytosine)-methyltransf ...
Bacillus amyloliquefaciens
Mol. Biol. (Mosk.)
35
35-44
2001
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485133
Bujnicki
Molecular evolution of DNA-(cy ...
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens H, Bacillus subtilis, Cenarchaeum symbiosum, Cenarchaeum symbiosum A, Cenarchaeum symbiosum B, Citrobacter freundii, Citrobacter freundii 4111, Citrobacter freundii RFL9, Curtobacterium albidum, Geobacillus stearothermophilus, Geobacillus stearothermophilus JN2091, Gordonia rubripertincta, Halobacterium phage phiH, Helicobacter pylori, Kocuria varians, Kocuria varians RFL19, Lactobacillus phage phig1e, Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicus, Methanothermobacter thermautotrophicus Z-250, Methanothermobacter wolfeii, Neisseria gonorrhoeae, Neisseria gonorrhoeae ME11, Proteus hauseri, Pseudomonas alcaligenes, Pseudomonas alcaligenes NCIB 986, Pyrococcus horikoshii, Pyrococcus horikoshii OT-3, Pyrococcus sp., Saccharopolyspora sp., Salmonella enterica subsp. enterica serovar Typhi, Salmonella enterica subsp. enterica serovar Typhi CT18, Serratia marcescens, Streptomyces caespitosus, Streptomyces fimbriatus, Trichormus variabilis, Xanthomonas campestris pv. cyanopsidis, Xanthomonas citri pv. malvacearum
Nucleic Acids Res.
27
4501-4509
1999
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88
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44
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44
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485135
Merkiene
A pair of single-strand and do ...
Brevibacillus centrosporus
Biol. Chem.
379
569-571
1998
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485129
O'Gara
Expression, purification, mass ...
Proteus vulgaris
Eur. J. Biochem.
247
1009-1018
1997
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485132
Adams
The PvuII DNA (cytosine-N4)-me ...
Proteus vulgaris
Biochemistry
36
8284-8292
1997
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485134
Gong
Structure of pvu II DNA-(cytos ...
Proteus vulgaris
Nucleic Acids Res.
25
2702-2715
1997
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485127
Roberts
Restriction enzymes and their ...
Bacteria
Nucleic Acids Res.
20
2167-2180
1992
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485126
Klimasauskas
Sequence motifs characteristic ...
Citrobacter freundii, Citrobacter freundii RFL9, Kocuria varians, Kocuria varians RFL19
Nucleic Acids Res.
17
9823-9832
1989
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4
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-
-
-
-
-
-
-
-
-
-
-
-
-
-