BRENDA - Enzyme Database
show all sequences of 2.1.1.111

Anthranilate N-methyltransferase, a branch-point enzyme of acridone biosynthesis

Rohde, B.; Hans, J.; Martens, S.; Baumert, A.; Hunziker, P.; Matern, U.; Plant J. 53, 541-553 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli, BL21 cells for induction of recombinant protein expression
Ruta graveolens
Engineering
Amino acid exchange
Commentary
Organism
N298E
generated by site-directed mutagenesis
Ruta graveolens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
S-adenosyl-L-methionine
N298E mutant; wild-type
Ruta graveolens
0.007
-
anthranilate
wild-type
Ruta graveolens
0.024
-
anthranilate
N298E mutant
Ruta graveolens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
73% relative enzyme activity in presence of 1.5 mM, significantly reduced
Ruta graveolens
Co2+
activity reduced in presence of 1.5 mM, 25% relative activity retained
Ruta graveolens
Cu2+
activity abolished in presence of 1.5 mM of
Ruta graveolens
Fe2+
activity nearly abolished in presence of 1.5 mM of
Ruta graveolens
Fe3+
activity abolished in presence of 1.5 mM of
Ruta graveolens
Mg2+
activity independent in presence of 1.5 mM of, 90% relative activity retained
Ruta graveolens
Zn2+
activity abolished in presence of 1.5 mM of
Ruta graveolens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
-
gel filtration, SDS-PAGE
Ruta graveolens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + anthranilate
Ruta graveolens
first specific step in acridone alkaloid biosynthesis
S-adenosyl-L-homocysteine + N-methylanthranilate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ruta graveolens
A9X7L0
line R-20
-
Purification (Commentary)
Commentary
Organism
gel filtration, SDS-PAGE, wild-type and mutant protein
Ruta graveolens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
carpel
lining the inside of
Ruta graveolens
-
cell suspension culture
crude extracts of total plants, elicited, 2 years old
Ruta graveolens
-
flower
high mRNA expression and enzyme activity
Ruta graveolens
-
leaf
moderate mRNA expression
Ruta graveolens
-
ovary
central vascular bundles of, around cup-like structures in the outer ovary wall representing secretory oil glands
Ruta graveolens
-
root
rhizodermis of older root parts, endodermis of younger root parts, high mRNA expression and enzyme activity
Ruta graveolens
-
stem
vascular region of secondary and primary shoots, moderate mRNA expression, high enzyme activity
Ruta graveolens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
in-gel digestion and peptide sequencing by mass spectrometry, Western Blot and phylogenetic analysis, assays conducted at 32°C in 73.5 mM glycine-NaOH buffer at pH 8.9, HPLC separation of assay extracts, list of compounds that are not accepted as substrate in assay
Ruta graveolens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + anthranilate
first specific step in acridone alkaloid biosynthesis
689543
Ruta graveolens
S-adenosyl-L-homocysteine + N-methylanthranilate
-
-
-
?
S-adenosyl-L-methionine + anthranilate
first specific step in alkaloid biosynthesis, substrate specificity determines the course of acridone biosynthesis
689543
Ruta graveolens
S-adenosyl-L-homocysteine + N-methylanthranilate
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
32
-
assay at
Ruta graveolens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
activity optimum at
Ruta graveolens
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli, BL21 cells for induction of recombinant protein expression
Ruta graveolens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
N298E
generated by site-directed mutagenesis
Ruta graveolens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
S-adenosyl-L-methionine
N298E mutant; wild-type
Ruta graveolens
0.007
-
anthranilate
wild-type
Ruta graveolens
0.024
-
anthranilate
N298E mutant
Ruta graveolens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
73% relative enzyme activity in presence of 1.5 mM, significantly reduced
Ruta graveolens
Co2+
activity reduced in presence of 1.5 mM, 25% relative activity retained
Ruta graveolens
Cu2+
activity abolished in presence of 1.5 mM of
Ruta graveolens
Fe2+
activity nearly abolished in presence of 1.5 mM of
Ruta graveolens
Fe3+
activity abolished in presence of 1.5 mM of
Ruta graveolens
Mg2+
activity independent in presence of 1.5 mM of, 90% relative activity retained
Ruta graveolens
Zn2+
activity abolished in presence of 1.5 mM of
Ruta graveolens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
-
gel filtration, SDS-PAGE
Ruta graveolens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + anthranilate
Ruta graveolens
first specific step in acridone alkaloid biosynthesis
S-adenosyl-L-homocysteine + N-methylanthranilate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
gel filtration, SDS-PAGE, wild-type and mutant protein
Ruta graveolens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
carpel
lining the inside of
Ruta graveolens
-
cell suspension culture
crude extracts of total plants, elicited, 2 years old
Ruta graveolens
-
flower
high mRNA expression and enzyme activity
Ruta graveolens
-
leaf
moderate mRNA expression
Ruta graveolens
-
ovary
central vascular bundles of, around cup-like structures in the outer ovary wall representing secretory oil glands
Ruta graveolens
-
root
rhizodermis of older root parts, endodermis of younger root parts, high mRNA expression and enzyme activity
Ruta graveolens
-
stem
vascular region of secondary and primary shoots, moderate mRNA expression, high enzyme activity
Ruta graveolens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
in-gel digestion and peptide sequencing by mass spectrometry, Western Blot and phylogenetic analysis, assays conducted at 32°C in 73.5 mM glycine-NaOH buffer at pH 8.9, HPLC separation of assay extracts, list of compounds that are not accepted as substrate in assay
Ruta graveolens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + anthranilate
first specific step in acridone alkaloid biosynthesis
689543
Ruta graveolens
S-adenosyl-L-homocysteine + N-methylanthranilate
-
-
-
?
S-adenosyl-L-methionine + anthranilate
first specific step in alkaloid biosynthesis, substrate specificity determines the course of acridone biosynthesis
689543
Ruta graveolens
S-adenosyl-L-homocysteine + N-methylanthranilate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
32
-
assay at
Ruta graveolens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
activity optimum at
Ruta graveolens
Other publictions for EC 2.1.1.111
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
689543
Rohde
Anthranilate N-methyltransfera ...
Ruta graveolens
Plant J.
53
541-553
2008
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1
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1
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3
-
7
1
1
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3
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1
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7
1
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2
-
1
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-
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1
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1
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1
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3
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7
1
1
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1
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7
1
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2
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1
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1
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485123
Maier
-
Partial purification and chara ...
Ruta graveolens
J. Plant Physiol.
145
1-6
1995
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-
-
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-
-
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2
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1
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1
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-
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1
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1
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-
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2
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1
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-
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-
1
-
-
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-
1
-
-
-
-
-
-
-
-
-
485122
Baumert
-
Increased accumulation of Acri ...
Ruta graveolens
J. Plant Physiol.
139
224-228
1991
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-
-
-
-
-
-
-
-
-
-
1
-
1
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2
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1
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2
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-
-
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-
-
-
485121
Baumert
-
Anthranilic acid-specific enzy ...
Choisya ternata, Ruta graveolens
Plant Physiol. Biochem.
28
587-592
1990
-
-
-
-
-
-
-
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-
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2
-
2
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4
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2
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2
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2
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4
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2
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2
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485120
Eilert
-
Elicitor induction of S-adenos ...
Ruta graveolens
Plant Cell Tissue Organ Cult.
18
1-18
1989
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1
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1
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1
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1
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2
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1
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1
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1
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1
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1
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