BRENDA - Enzyme Database show
show all sequences of 2.1.1.108

A methyltransferase forsynthesis of the phytoalexin 6-methoxymellein in carrot cells

Kurosaki, F.; Nishi, A.; FEBS Lett. 227, 183-186 (1988)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-Chloroethylphosphonic acid
elicitor, the activity increases after a 2 day lag period and attains a maximum 3 days after the addition, then decreases gradually
Daucus carota
uronide
elicitor
Daucus carota
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + 6-hydroxymellein
Daucus carota
enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue
S-adenosyl-L-homocysteine + 6-methoxymellein
-
Daucus carota
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Daucus carota
-
treated with 2-chloroethylphosphonic acid; treated with fungal elicitors
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Daucus carota
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no substrates: umbelliferone, scopoletin
485093
Daucus carota
?
-
-
-
-
additional information
no substrates: 3,4-dehydro-6-methoxymellein, 6-methoxymellein, esculetin
485093
Daucus carota
?
-
-
-
-
S-adenosyl-L-methionine + 3,4-dehydro-6-hydroxymellein
-
485093
Daucus carota
S-adenosyl-L-homocysteine + 3,4-dehydro-6-methoxymellein
-
485093
Daucus carota
?
S-adenosyl-L-methionine + 6-hydroxymellein
-
485093
Daucus carota
S-adenosyl-L-homocysteine + 6-methoxymellein
-
485093
Daucus carota
?
S-adenosyl-L-methionine + 6-hydroxymellein
enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue
485093
Daucus carota
S-adenosyl-L-homocysteine + 6-methoxymellein
-
485093
Daucus carota
?
S-adenosyl-L-methionine + 6-hydroxymellein + 3,4-dehydro-6-hydroxymellein
-
485093
Daucus carota
S-adenosyl-L-homocysteine + 6-methoxymellein + 3,4-dehydro-6-methoxymellein
-
485093
Daucus carota
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Daucus carota
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-Chloroethylphosphonic acid
elicitor, the activity increases after a 2 day lag period and attains a maximum 3 days after the addition, then decreases gradually
Daucus carota
uronide
elicitor
Daucus carota
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + 6-hydroxymellein
Daucus carota
enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue
S-adenosyl-L-homocysteine + 6-methoxymellein
-
Daucus carota
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Daucus carota
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no substrates: umbelliferone, scopoletin
485093
Daucus carota
?
-
-
-
-
additional information
no substrates: 3,4-dehydro-6-methoxymellein, 6-methoxymellein, esculetin
485093
Daucus carota
?
-
-
-
-
S-adenosyl-L-methionine + 3,4-dehydro-6-hydroxymellein
-
485093
Daucus carota
S-adenosyl-L-homocysteine + 3,4-dehydro-6-methoxymellein
-
485093
Daucus carota
?
S-adenosyl-L-methionine + 6-hydroxymellein
-
485093
Daucus carota
S-adenosyl-L-homocysteine + 6-methoxymellein
-
485093
Daucus carota
?
S-adenosyl-L-methionine + 6-hydroxymellein
enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue
485093
Daucus carota
S-adenosyl-L-homocysteine + 6-methoxymellein
-
485093
Daucus carota
?
S-adenosyl-L-methionine + 6-hydroxymellein + 3,4-dehydro-6-hydroxymellein
-
485093
Daucus carota
S-adenosyl-L-homocysteine + 6-methoxymellein + 3,4-dehydro-6-methoxymellein
-
485093
Daucus carota
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Daucus carota
Other publictions for EC 2.1.1.108
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
485095
Kurosaki
-
Partial purification and chara ...
Daucus carota
Phytochemistry
41
1023-1027
1996
-
-
-
-
-
-
10
-
-
-
1
2
-
1
-
-
1
1
-
-
1
2
3
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
10
2
-
-
-
1
2
-
-
-
1
-
-
1
2
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
485096
Marinelli
-
Elicitor induction of enzyme a ...
Daucus carota
Phytochemistry
35
1457-1460
1994
2
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485094
Kurosaki
-
Biosynthesis of dihydroisocoum ...
Daucus carota
Phytochemistry
28
1843-1845
1989
1
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485093
Kurosaki
-
A methyltransferase forsynthes ...
Daucus carota
FEBS Lett.
227
183-186
1988
2
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
6
-
1
-
-
-
-
-
-
-
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-
2
-
-
-
-
-
-
-
-
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-
1
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-
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1
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-
6
-
1
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