BRENDA - Enzyme Database show
show all sequences of 2.1.1.107

Purification, characterization, and molecular cloning of S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase from Methanobacterium ivanovii

Blanche, F.; Robin, C.; Couder, M.; Faucher, D.; Cauchois, L.; Cameron, B.; Crouzet, J.; J. Bacteriol. 173, 4637-4645 (1991)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli and Pseudomonas denitrificans
Methanobacterium ivanovii
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no substrate inhibition at uroporphyrinogen III concentrations of up to 0.02 mM
Methanobacterium ivanovii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.052
-
uroporphyrinogen III
pH 7.7, 30°C
Methanobacterium ivanovii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
2 * 29000, SDS-PAGE
Methanobacterium ivanovii
58200
-
gel filtration
Methanobacterium ivanovii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanobacterium ivanovii
P29564
-
-
Purification (Commentary)
Commentary
Organism
-
Methanobacterium ivanovii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.026
-
pH 7.7, 30°C
Methanobacterium ivanovii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 S-adenosyl-L-methionine + uroporphyrinogen III
-
727722
Methanobacterium ivanovii
2 S-adenosyl-L-homocysteine + precorrin-2
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 29000, SDS-PAGE
Methanobacterium ivanovii
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli and Pseudomonas denitrificans
Methanobacterium ivanovii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no substrate inhibition at uroporphyrinogen III concentrations of up to 0.02 mM
Methanobacterium ivanovii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.052
-
uroporphyrinogen III
pH 7.7, 30°C
Methanobacterium ivanovii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
2 * 29000, SDS-PAGE
Methanobacterium ivanovii
58200
-
gel filtration
Methanobacterium ivanovii
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methanobacterium ivanovii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.026
-
pH 7.7, 30°C
Methanobacterium ivanovii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 S-adenosyl-L-methionine + uroporphyrinogen III
-
727722
Methanobacterium ivanovii
2 S-adenosyl-L-homocysteine + precorrin-2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 29000, SDS-PAGE
Methanobacterium ivanovii
Other publictions for EC 2.1.1.107
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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747676
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Vannini
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286
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1
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3
1
1
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1
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1
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706702
Araya
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Geobacillus stearothermophilus, Geobacillus stearothermophilus V
Res. Microbiol.
160
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1
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1
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1
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2
1
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1
1
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676956
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Zea mays
Protein Expr. Purif.
46
40-46
2006
-
1
1
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1
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-
-
-
-
3
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3
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1
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1
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660612
Rehse
Structure of a closed-form uro ...
Thermus thermophilus
Acta Crystallogr. Sect. D
61
913-919
2005
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662638
Vevodova
Structure/function studies on ...
Pseudomonas denitrificans (nomen rejiciendum)
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419-433
2004
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6
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485092
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The role of Saccharomyces cere ...
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338
701-708
1999
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6
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485091
Woodcock
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Effect of mutations in the tra ...
Escherichia coli
Biochem. J.
330
121-129
1998
-
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1
-
7
-
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7
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485090
Leustek
Siroheme biosynthesis in highe ...
Arabidopsis thaliana
J. Biol. Chem.
272
2744-2752
1997
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1
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5
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485089
Sakakibara
Isolation and characterization ...
Zea mays
Plant J.
10
883-892
1996
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1
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485087
Sattler
Cloning, sequencing, and expre ...
Propionibacterium freudenreichii
J. Bacteriol.
177
1564-1569
1995
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1
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485088
Chou
Reddish Escherichia coli cells ...
Geobacillus stearothermophilus
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59
1817-1824
1995
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1
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1
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727722
Blanche
Purification, characterization ...
Methanobacterium ivanovii
J. Bacteriol.
173
4637-4645
1991
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1
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1
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485083
Warren
-
Uroporpyrinogen III methylase ...
Escherichia coli
J. Am. Chem. Soc.
112
5343-5345
1990
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1
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485084
Warren
The Escherichia coli cysG gene ...
Escherichia coli
Biochem. J.
265
725-729
1990
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1
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3
1
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1
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485086
Crouzet
Nucleotide sequence of a Pseud ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
172
5968-5979
1990
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1
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1
2
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2
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1
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2
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4
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485085
Blanche
Purification and characterizat ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
171
4222-4231
1989
-
-
1
-
-
-
3
5
-
1
2
1
-
2
-
-
-
-
-
-
1
1
8
1
-
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-
1
1
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1
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1
-
-
-
-
-
3
1
5
-
1
2
1
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1
1
8
1
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1
1
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