Cloned (Comment) | Organism |
---|---|
recombinant expression of C-terminally His-tagged NirE in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH10B | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a nirE transposon mutant, which is not complemented by native cobA encoding the SAM-dependent uroporphyrinogen III methyltransferase involved in cobalamin formation, overview | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | product inhibition at concentrations above 0.002 mM | Pseudomonas aeruginosa | |
uroporphyrinogen III | substrate inhibition at concentrations above 0.017 mM | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, SDS-PAGE | Pseudomonas aeruginosa |
60000 | - |
gel filtration | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uroporphyrinogen III + S-adenosyl-L-methionine | Pseudomonas aeruginosa | - |
precorrin-2 + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene nirE | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged NirE from Escherichia coli by metal chelate chromatography and gel filtration to homogeneity | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000044 | - |
recombinant enzyme | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | at high enzyme concentrations NirE catalyzes an overmethylation of uroporphyrinogen III, resulting in the formation of trimethylpyrrocorphin | Pseudomonas aeruginosa | ? | - |
? | |
uroporphyrinogen III + S-adenosyl-L-methionine | - |
Pseudomonas aeruginosa | precorrin-2 + S-adenosyl-L-homocysteine | - |
? | |
uroporphyrinogen III + S-adenosyl-L-methionine | NirE binds S-adenosyl-L-methionine, although not with the same avidity as reported for other SAM-dependent uroporphyrinogen III methyltransferases involved in siroheme and cobalamin biosynthesis | Pseudomonas aeruginosa | precorrin-2 + S-adenosyl-L-homocysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 30000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
NirE | - |
Pseudomonas aeruginosa |
SAM-dependent uroporphyrinogen III methyltransferase | - |
Pseudomonas aeruginosa |
SUMT | - |
Pseudomonas aeruginosa |
uroporphyrinogen III methyltransferase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | dependent on | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is important in the heme d1 biosynthesis, the essential prosthetic group of the dissimilatory nitrite reductase cytochrome cd1 requiring the methylation of the tetrapyrrole precursor uroporphyrinogen III at positions C-2 and C-7, pathway overview | Pseudomonas aeruginosa |