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Literature summary for 2.1.1.107 extracted from

  • Storbeck, S.; Walther, J.; Mueller, J.; Parmar, V.; Schiebel, H.M.; Kemken, D.; Duelcks, T.; Warren, M.J.; Layer, G.
    The Pseudomonas aeruginosa nirE gene encodes the S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase required for heme d1 biosynthesis (2009), FEBS J., 276, 5973-5982.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of C-terminally His-tagged NirE in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH10B Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information construction of a nirE transposon mutant, which is not complemented by native cobA encoding the SAM-dependent uroporphyrinogen III methyltransferase involved in cobalamin formation, overview Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine product inhibition at concentrations above 0.002 mM Pseudomonas aeruginosa
uroporphyrinogen III substrate inhibition at concentrations above 0.017 mM Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
2 * 30000, SDS-PAGE Pseudomonas aeruginosa
60000
-
gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uroporphyrinogen III + S-adenosyl-L-methionine Pseudomonas aeruginosa
-
precorrin-2 + S-adenosyl-L-homocysteine
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
gene nirE
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged NirE from Escherichia coli by metal chelate chromatography and gel filtration to homogeneity Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000044
-
recombinant enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information at high enzyme concentrations NirE catalyzes an overmethylation of uroporphyrinogen III, resulting in the formation of trimethylpyrrocorphin Pseudomonas aeruginosa ?
-
?
uroporphyrinogen III + S-adenosyl-L-methionine
-
Pseudomonas aeruginosa precorrin-2 + S-adenosyl-L-homocysteine
-
?
uroporphyrinogen III + S-adenosyl-L-methionine NirE binds S-adenosyl-L-methionine, although not with the same avidity as reported for other SAM-dependent uroporphyrinogen III methyltransferases involved in siroheme and cobalamin biosynthesis Pseudomonas aeruginosa precorrin-2 + S-adenosyl-L-homocysteine
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 30000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
NirE
-
Pseudomonas aeruginosa
SAM-dependent uroporphyrinogen III methyltransferase
-
Pseudomonas aeruginosa
SUMT
-
Pseudomonas aeruginosa
uroporphyrinogen III methyltransferase
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine dependent on Pseudomonas aeruginosa

General Information

General Information Comment Organism
metabolism the enzyme is important in the heme d1 biosynthesis, the essential prosthetic group of the dissimilatory nitrite reductase cytochrome cd1 requiring the methylation of the tetrapyrrole precursor uroporphyrinogen III at positions C-2 and C-7, pathway overview Pseudomonas aeruginosa