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Literature summary for 2.1.1.106 extracted from

  • Benjdia, A.; Pierre, S.; Gherasim, C.; Guillot, A.; Carmona, M.; Amara, P.; Banerjee, R.; Berteau, O.
    The thiostrepton A tryptophan methyltransferase TsrM catalyses a cob(II)alamin-dependent methyl transfer reaction (2015), Nat. Commun., 6, 8377 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.106

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces laurentii

Organism

Organism UniProt Comment Textmining
Streptomyces laurentii C0JRZ9
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.6
-
 substrate L-5-fluoro-tryptophan, pH 7.5, 25°C Streptomyces laurentii
1.2
-
 substrate L-5-hydroxy-tryptophan, pH 7.5, 25°C Streptomyces laurentii
1.3
-
 substrate L-5-methyl-tryptophan, pH 7.5, 25°C Streptomyces laurentii
3.5
-
 cofactor hydroxycob(III)alamin, in presence of dithiothreitol, pH 7.5, 25°C Streptomyces laurentii
3.9
-
 cofactor methylcob(III)alamin, pH 7.5, 25°C Streptomyces laurentii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information TsrM does not catalyse substrate H-atom abstraction but probably exploits a radical-based C-methylation mechanism. TsrM catalyzes methyl transfer to all Trp derivatives tested that are substituted on the indole ring Streptomyces laurentii ?
-
-
S-adenosyl-L-methionine + 3-methylindole
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + D-tryptophan
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + D-2-methyltryptophan
-
 ?
S-adenosyl-L-methionine + L-5-fluoro-tryptophan
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + L-5-fluoro-2-methyltryptophan
-
 ?
S-adenosyl-L-methionine + L-5-hydroxy-tryptophan
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + L-5-hydroxy-2-methyltryptophan
-
 ?
S-adenosyl-L-methionine + L-5-methyl-tryptophan
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + L-2,5-dimethyltryptophan
-
 ?
S-adenosyl-L-methionine + L-tryptophan
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + L-2-methyltryptophan
-
 ?
S-adenosyl-L-methionine + melatonin
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + serotonin
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + ?
-
 ?

Synonyms

Synonyms Comment Organism
thiostrepton A tryptophan methyltransferase
-
 Streptomyces laurentii

Cofactor

Cofactor Comment Organism Structure
5'-deoxyadenosylcobalamin 5'-deoxyadenosylcob(III)alamin may substitute for methylcob(III)alamin, albeit only in the presence of light Streptomyces laurentii
hydroxycobalamin
-
 Streptomyces laurentii
methylcobalamin probable physiological enzyme cofactor methylcob(III)alamin, and cob(II)alamin rather than cob(I)alamin is a key reaction intermediate Streptomyces laurentii
methylcobinamide about 5% of the activity with methylcob(III)alamin Streptomyces laurentii