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Literature summary for 2.1.1.104 extracted from

  • Zhang, X.S.; Ni, R.; Wang, P.Y.; Zhu, T.T.; Sun, C.J.; Lou, H.X.; Cheng, A.X.
    Isolation and functional characterization of two caffeoyl coenzyme A 3-O-methyltransferases from the fern species Polypodiodes amoena (2019), Plant Physiol. Biochem., 136, 169-177 .
    View publication on PubMed
Search for more literature for 2.1.1.104

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells and Nicotiana benthamiana Goniophlebium amoenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04774
-
 caffeoyl-CoA isoform CCoAOMT1, at pH 7.5 and 37°C Goniophlebium amoenum
0.04895
-
 quercetin isoform CCoAOMT1, at pH 7.5 and 37°C Goniophlebium amoenum
0.05114
-
 caffeoyl-CoA isoform CCoAOMT2, at pH 7.5 and 37°C Goniophlebium amoenum
0.05465
-
 eriodictyol isoform CCoAOMT2, at pH 7.5 and 37°C Goniophlebium amoenum
0.09159
-
 quercetin isoform CCoAOMT2, at pH 7.5 and 37°C Goniophlebium amoenum
0.09636
-
 eriodictyol isoform CCoAOMT1, at pH 7.5 and 37°C Goniophlebium amoenum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Goniophlebium amoenum 5737
-

Organism

Organism UniProt Comment Textmining
Goniophlebium amoenum A0A4Y5R9F4
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Sefinose column chromatography Goniophlebium amoenum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00379
-
 isoform CCoAOMT1, with 5-hydroxyconiferaldehyde as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.00664
-
 isoform CCoAOMT2, with caffeoyl aldehyde as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.00804
-
 isoform CCoAOMT2, with caffeoyl alcohol as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.01107
-
 isoform CCoAOMT2, with 5-hydroxyconiferaldehyde as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.01195
-
 isoform CCoAOMT2, with baicalein as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.01332
-
 isoform CCoAOMT1, with caffeoyl aldehyde as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.01476
-
 isoform CCoAOMT2, with scutellarein as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.018
-
 isoform CCoAOMT1, with eriodictyol as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.02313
-
 isoform CCoAOMT1, with scutellarein as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.02643
-
 isoform CCoAOMT1, with baicalein as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.02719
-
 isoform CCoAOMT2, with caffeoyl-CoA as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.03484
-
 isoform CCoAOMT1, with caffeoyl-CoA as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.03742
-
 isoform CCoAOMT2, with luteolin as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.04316
-
 isoform CCoAOMT2, with quercetin as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.04848
-
 isoform CCoAOMT2, with eriodictyol as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.05571
-
 isoform CCoAOMT1, with luteolin as substrate, at pH 7.5 and 37°C Goniophlebium amoenum
0.05677
-
 isoform CCoAOMT1, with quercetin as substrate, at pH 7.5 and 37°C Goniophlebium amoenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is not able to methylate caffeic acid, 5-hydroxyferulic acid, 5-hydroxyconiferyl alcohol, kaempferol and naringenin Goniophlebium amoenum ?
-
-
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
-
 Goniophlebium amoenum ?
-
 ?
S-adenosyl-L-methionine + baicalein
-
 Goniophlebium amoenum ?
-
 ?
S-adenosyl-L-methionine + caffeoyl alcohol
-
 Goniophlebium amoenum ?
-
 ?
S-adenosyl-L-methionine + caffeoyl aldehyde
-
 Goniophlebium amoenum ?
-
 ?
S-adenosyl-L-methionine + caffeoyl-CoA
-
 Goniophlebium amoenum S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
S-adenosyl-L-methionine + eriodictyol
-
 Goniophlebium amoenum ?
-
 ?
S-adenosyl-L-methionine + luteolin
-
 Goniophlebium amoenum ?
-
 ?
S-adenosyl-L-methionine + quercetin
-
 Goniophlebium amoenum ?
-
 ?
S-adenosyl-L-methionine + scutellarein
-
 Goniophlebium amoenum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 28750, isoform CCoAOMT1, calculated from amino acid sequence Goniophlebium amoenum
? x * 32280, isoform CCoAOMT2, calculated from amino acid sequence Goniophlebium amoenum

Synonyms

Synonyms Comment Organism
caffeoyl coenzyme A 3-O-methyltransferase
-
 Goniophlebium amoenum
CCoAOMT1 isoform Goniophlebium amoenum
CCoAOMT2 isoform Goniophlebium amoenum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 caffeoyl-CoA isoform CCoAOMT1, at pH 7.5 and 37°C Goniophlebium amoenum
0.04
-
 caffeoyl-CoA isoform CCoAOMT2, at pH 7.5 and 37°C Goniophlebium amoenum
0.05
-
 eriodictyol isoform CCoAOMT1, at pH 7.5 and 37°C Goniophlebium amoenum
0.05
-
 quercetin isoform CCoAOMT2, at pH 7.5 and 37°C Goniophlebium amoenum
0.13
-
 eriodictyol isoform CCoAOMT2, at pH 7.5 and 37°C Goniophlebium amoenum
0.14
-
 quercetin isoform CCoAOMT1, at pH 7.5 and 37°C Goniophlebium amoenum

General Information

General Information Comment Organism
physiological function the enzyme contributes to lignin synthesis Goniophlebium amoenum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.5303
-
 quercetin isoform CCoAOMT2, at pH 7.5 and 37°C Goniophlebium amoenum
0.5309
-
 eriodictyol isoform CCoAOMT1, at pH 7.5 and 37°C Goniophlebium amoenum
0.7388
-
 caffeoyl-CoA isoform CCoAOMT2, at pH 7.5 and 37°C Goniophlebium amoenum
0.8783
-
 caffeoyl-CoA isoform CCoAOMT1, at pH 7.5 and 37°C Goniophlebium amoenum
2.4386
-
 eriodictyol isoform CCoAOMT2, at pH 7.5 and 37°C Goniophlebium amoenum
2.7637
-
 quercetin isoform CCoAOMT1, at pH 7.5 and 37°C Goniophlebium amoenum