Cloned (Comment) | Organism |
---|---|
gene At4g26220, recombinant expression of the enzyme in yeast cells, coexpression with enzymes S-adenosyl-L-methionine synthase (SAMS)1, SAMS2, SAMS3, SAMS4, methionine synthase (MS)1, MS2, S-adenosyl-L-homocysteine hydrolase (SAHH)1, and SAHH2 for the yeast two-hybrid interaction analysis, overview. Quantitative real-time reverse transcript-PCR expression analysis | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Arabidopsis thaliana | 5618 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | enzyme CCoAOMT7 binds to S-adenosyl-L-homocysteine hydrolase (SAHH) and S-adenosyl-L-methionine synthases (SAMSs) in vivo | ? | - |
- |
|
additional information | Arabidopsis thaliana Col-0 | enzyme CCoAOMT7 binds to S-adenosyl-L-homocysteine hydrolase (SAHH) and S-adenosyl-L-methionine synthases (SAMSs) in vivo | ? | - |
- |
|
S-adenosyl-L-methionine + caffeoyl-CoA | Arabidopsis thaliana | - |
S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
S-adenosyl-L-methionine + caffeoyl-CoA | Arabidopsis thaliana Col-0 | - |
S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
S-adenosyl-L-methionine + luteolin | Arabidopsis thaliana | - |
S-adenosyl-L-homocysteine + scoparol | - |
? | |
S-adenosyl-L-methionine + luteolin | Arabidopsis thaliana Col-0 | - |
S-adenosyl-L-homocysteine + scoparol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9C5D7 | - |
- |
Arabidopsis thaliana Col-0 | Q9C5D7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme CCoAOMT7 binds to S-adenosyl-L-homocysteine hydrolase (SAHH) and S-adenosyl-L-methionine synthases (SAMSs) in vivo | Arabidopsis thaliana | ? | - |
- |
|
additional information | CCoAOMT7 in Arabidopsis thaliana shows a strong preference to methylate para-position of flavanones and dihydroflavonols, and also a relatively lower activity on caffeoyl-CoA using S-adenosyl-L-methionine (SAM or AdoMet) as methyl donor to produce feruloyl-CoA. CCoAOMT7 also shows methyltransferase activity on caffeoyl CoA, although it shows the highest activity on luteolin, a kind of flavonoids | Arabidopsis thaliana | ? | - |
- |
|
additional information | enzyme CCoAOMT7 binds to S-adenosyl-L-homocysteine hydrolase (SAHH) and S-adenosyl-L-methionine synthases (SAMSs) in vivo | Arabidopsis thaliana Col-0 | ? | - |
- |
|
additional information | CCoAOMT7 in Arabidopsis thaliana shows a strong preference to methylate para-position of flavanones and dihydroflavonols, and also a relatively lower activity on caffeoyl-CoA using S-adenosyl-L-methionine (SAM or AdoMet) as methyl donor to produce feruloyl-CoA. CCoAOMT7 also shows methyltransferase activity on caffeoyl CoA, although it shows the highest activity on luteolin, a kind of flavonoids | Arabidopsis thaliana Col-0 | ? | - |
- |
|
S-adenosyl-L-methionine + caffeoyl-CoA | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
S-adenosyl-L-methionine + caffeoyl-CoA | - |
Arabidopsis thaliana Col-0 | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
S-adenosyl-L-methionine + luteolin | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + scoparol | - |
? | |
S-adenosyl-L-methionine + luteolin | - |
Arabidopsis thaliana Col-0 | S-adenosyl-L-homocysteine + scoparol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
caffeoyl CoA methyltransferase | - |
Arabidopsis thaliana |
caffeoyl CoA methyltransferase 7 | - |
Arabidopsis thaliana |
CCoAOMT | - |
Arabidopsis thaliana |
CCoAOMT7 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | interruptions of endogenous SAHH1 by artificial miRNA or SAMSs by T-DNA insertion significantly reduce ferulate contents in the stem cell wall | Arabidopsis thaliana |
physiological function | ferulate is methylated from caffeoyl-CoA using S-adenosyl-L-methionine (SAM) as methyl donor catalyzed by caffeoyl CoA methyltransferase (CCoAOMT). Arabidopsis thaliana CCoAOMT7 contributes to ferulate content in the stem cell wall. CCoAOMT7 further binds to S-adenosyl-L-homocysteine hydrolase (SAHH), a critical step in SAM synthesis to release feedback suppression on CCoAOMT. CCoAOMT7 also binds S-adenosyl-L-methionine synthases (SAMSs) in vivo, which is mediated by SAHH1. Enzyme CCoAOMT7 is required for cell wall-bound ferulic esters | Arabidopsis thaliana |