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Literature summary for 2.1.1.104 extracted from

  • Day, A.; Neutelings, G.; Nolin, F.; Grec, S.; Habrant, A.; Cronier, D.; Maher, B.; Rolando, C.; David, H.; Chabbert, B.; Hawkins, S.
    Caffeoyl coenzyme A O-methyltransferase down-regulation is associated with modifications in lignin and cell-wall architecture in flax secondary xylem (2009), Plant Physiol. Biochem., 47, 9-19.
    View publication on PubMed
Search for more literature for 2.1.1.104

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Linum usitatissimum

Protein Variants

Protein Variants Comment Organism
additional information enzyme downregulation leads to reduced lignin quantity a modified syringyl/guaiacyl lignin monomer ratio in the non-condensed lignin fraction, together with altered xylem organization, reduced cell-wall thickness and the appearance of an irregular xylem phenotype, phentypes of two independently down-regulated lines, overview Linum usitatissimum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
 caffeoyl-CoA recombinant enzyme, 30°C Linum usitatissimum
0.039
-
 caffeic acid recombinant enzyme, 30°C Linum usitatissimum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27900
-
 x * 27900, about, sequence calculation Linum usitatissimum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Linum usitatissimum CCoAOMT down-regulation induces changes in xylem cell-wall structure and the lignin fractions ?
-
 ?
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA Linum usitatissimum
-
 S-adenosyl-L-homocysteine + sinapoyl-CoA
-
 ?
S-adenosyl-L-methionine + caffeic acid Linum usitatissimum
-
 S-adenosyl-L-homocysteine + ferulic acid
-
 ?
S-adenosyl-L-methionine + caffeoyl-CoA Linum usitatissimum
-
 S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Linum usitatissimum Q4JHB1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3), removal of the His-tag Linum usitatissimum

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Linum usitatissimum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information CCoAOMT down-regulation induces changes in xylem cell-wall structure and the lignin fractions Linum usitatissimum ?
-
 ?
additional information flax CCoAOMT possesses a small, but probably significant 5' methylating activity, in addition to a more usual 3' methylating activity Linum usitatissimum ?
-
 ?
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA
-
 Linum usitatissimum S-adenosyl-L-homocysteine + sinapoyl-CoA
-
 ?
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA 3' methylating activity Linum usitatissimum S-adenosyl-L-homocysteine + sinapoyl-CoA
-
 ?
S-adenosyl-L-methionine + caffeic acid
-
 Linum usitatissimum S-adenosyl-L-homocysteine + ferulic acid
-
 ?
S-adenosyl-L-methionine + caffeoyl-CoA
-
 Linum usitatissimum S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
S-adenosyl-L-methionine + caffeoyl-CoA preferred substrate, 3' methylating activity Linum usitatissimum S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 27900, about, sequence calculation Linum usitatissimum

Synonyms

Synonyms Comment Organism
caffeoyl coenzyme A O-methyltransferase
-
 Linum usitatissimum
CCoAOMT
-
 Linum usitatissimum
More the enzyme belongs to the O-methyltransferase superfamily Linum usitatissimum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Linum usitatissimum

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Linum usitatissimum

pI Value

Organism Comment pI Value Maximum pI Value
Linum usitatissimum sequence calculation
-
 5.3

General Information

General Information Comment Organism
metabolism the enzyme catalyzes central essential reactions in the lignin biosynthesis, pathway overview Linum usitatissimum