BRENDA - Enzyme Database show
show all sequences of 2.1.1.104

Cation dependent O-methyltransferases from rice

Lee, Y.; Kim, B.; Chong, Y.; Lim, Y.; Ahn, J.; Planta 227, 641-647 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
OMT-15 and OMT-17 are expressed in Escherichia coli BL21(DE3) cells as glutathione S-transferase fusion proteins
Oryza sativa
Engineering
Amino acid exchange
Commentary
Organism
D168L
the mutation abolishes the enzyme activity of OMT-15
Oryza sativa
D194L
the mutation abolishes the enzyme activity of OMT-15
Oryza sativa
D209L
the mutation abolishes the enzyme activity of OMT-17
Oryza sativa
D234L
the mutation abolishes the enzyme activity of OMT-17
Oryza sativa
E69L
the mutation results in about 14% loss of enzyme activity in OMT-15 and 40% loss of activity in OMT-17
Oryza sativa
N195I
the mutation abolishes the enzyme activity of OMT-15
Oryza sativa
N235I
the mutation abolishes the enzyme activity of OMT-17
Oryza sativa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.072
-
caffeoyl-CoA
OMT-17, in 10 mM Tris-HCl buffer (pH 7.5), at 37C
Oryza sativa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
Co2+ results in recovery of 82% of OMT-15 and 41% of OMT-17 activity compared to the addition of Mg2+
Oryza sativa
Mg2+
dependent on
Oryza sativa
Mn2+
the addition of Mn2+ restores 74% of OMT-15 activity and 69% of OMT-17 compared to the addition of Mg2+
Oryza sativa
Zn2+
Zn2+ results in recovery of less than 10% of OMT-15 and OMT-17 activity compared to the addition of Mg2+
Oryza sativa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27800
-
OMT-15, calculated from amino acid sequence
Oryza sativa
30600
-
recombinant OMT-17, SDS-PAGE
Oryza sativa
31100
-
recombinant OMT-15, SDS-PAGE
Oryza sativa
32000
-
OMT-17, calculated from amino acid sequence
Oryza sativa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryza sativa
-
-
-
Purification (Commentary)
Commentary
Organism
glutathione Sepharose 4 column chromatography
Oryza sativa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
OMT-15 is expressed higher in stem and roots than in other tissues
Oryza sativa
-
stem
OMT-17 is expressed in stems only, OMT-15 is expressed higher in stem and roots than in other tissues
Oryza sativa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
OMT-15 and -17 cannot utilize naringenin, apigenin, or kaempferol as substrates, taxifolin and eriodictyol do not serve as methyl acceptors
700855
Oryza sativa
?
-
-
-
-
S-adenosyl-L-methionine + 5-hydroxyferulic acid
OMT-15 shows 25% activity and OMT-17 shows 18% activity compared to myricetin
700855
Oryza sativa
?
-
-
-
?
S-adenosyl-L-methionine + 7,8-dihydroxyflavone
OMT-15 shows 102% activity and OMT-17 shows 143% activity compared to myricetin
700855
Oryza sativa
?
-
-
-
?
S-adenosyl-L-methionine + caffeoyl-CoA
OMT-15 shows 100% activity and OMT-17 shows 58% activity compared to myricetin
700855
Oryza sativa
S-adenosyl-L-homocysteine + ?
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
OMT-15 and OMT-17 are expressed in Escherichia coli BL21(DE3) cells as glutathione S-transferase fusion proteins
Oryza sativa
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D168L
the mutation abolishes the enzyme activity of OMT-15
Oryza sativa
D194L
the mutation abolishes the enzyme activity of OMT-15
Oryza sativa
D209L
the mutation abolishes the enzyme activity of OMT-17
Oryza sativa
D234L
the mutation abolishes the enzyme activity of OMT-17
Oryza sativa
E69L
the mutation results in about 14% loss of enzyme activity in OMT-15 and 40% loss of activity in OMT-17
Oryza sativa
N195I
the mutation abolishes the enzyme activity of OMT-15
Oryza sativa
N235I
the mutation abolishes the enzyme activity of OMT-17
Oryza sativa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.072
-
caffeoyl-CoA
OMT-17, in 10 mM Tris-HCl buffer (pH 7.5), at 37C
Oryza sativa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
Co2+ results in recovery of 82% of OMT-15 and 41% of OMT-17 activity compared to the addition of Mg2+
Oryza sativa
Mg2+
dependent on
Oryza sativa
Mn2+
the addition of Mn2+ restores 74% of OMT-15 activity and 69% of OMT-17 compared to the addition of Mg2+
Oryza sativa
Zn2+
Zn2+ results in recovery of less than 10% of OMT-15 and OMT-17 activity compared to the addition of Mg2+
Oryza sativa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27800
-
OMT-15, calculated from amino acid sequence
Oryza sativa
30600
-
recombinant OMT-17, SDS-PAGE
Oryza sativa
31100
-
recombinant OMT-15, SDS-PAGE
Oryza sativa
32000
-
OMT-17, calculated from amino acid sequence
Oryza sativa
Purification (Commentary) (protein specific)
Commentary
Organism
glutathione Sepharose 4 column chromatography
Oryza sativa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
OMT-15 is expressed higher in stem and roots than in other tissues
Oryza sativa
-
stem
OMT-17 is expressed in stems only, OMT-15 is expressed higher in stem and roots than in other tissues
Oryza sativa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
OMT-15 and -17 cannot utilize naringenin, apigenin, or kaempferol as substrates, taxifolin and eriodictyol do not serve as methyl acceptors
700855
Oryza sativa
?
-
-
-
-
S-adenosyl-L-methionine + 5-hydroxyferulic acid
OMT-15 shows 25% activity and OMT-17 shows 18% activity compared to myricetin
700855
Oryza sativa
?
-
-
-
?
S-adenosyl-L-methionine + 7,8-dihydroxyflavone
OMT-15 shows 102% activity and OMT-17 shows 143% activity compared to myricetin
700855
Oryza sativa
?
-
-
-
?
S-adenosyl-L-methionine + caffeoyl-CoA
OMT-15 shows 100% activity and OMT-17 shows 58% activity compared to myricetin
700855
Oryza sativa
S-adenosyl-L-homocysteine + ?
-
-
-
?
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.000503
-
caffeoyl-CoA
OMT-17, in 10 mM Tris-HCl buffer (pH 7.5), at 37C
Oryza sativa
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.000503
-
caffeoyl-CoA
OMT-17, in 10 mM Tris-HCl buffer (pH 7.5), at 37C
Oryza sativa
Other publictions for EC 2.1.1.104
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734588
Zhang
Cloning of a caffeoyl-coenzyme ...
Camellia sinensis
J. Zhejiang Univ. Sci. B
16
103-112
2015
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734862
Brandt
A catalytic triad - Lys-Asn-As ...
Mesembryanthemum crystallinum
Phytochemistry
113
130-139
2015
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7
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734993
Ma
Biochemical characterization o ...
Triticum aestivum
Planta
242
113-122
2015
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1
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733542
Naaz
Structure-function analyses an ...
Medicago sativa
Biotechnol. Appl. Biochem.
60
170-189
2013
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733869
Wils
A single amino acid determines ...
Arabidopsis thaliana
FEBS Lett.
587
683-689
2013
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3
733942
Li
Downregulation of caffeoyl-CoA ...
Zea mays
Genet. Mol. Biol.
36
540-546
2013
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720719
Ghosh
-
Comparative transcriptional an ...
Hibiscus cannabinus
Plant OMICS
5
184-193
2012
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1
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720797
Fellenberg
The role of CCoAOMT1 and COMT1 ...
Arabidopsis thaliana
Planta
236
51-61
2012
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734664
Zhang
Harvest-inducibility of the pr ...
Medicago sativa
Mol. Biol. Rep.
39
2489-2495
2012
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720716
Widiez
Functional characterization of ...
Vanilla planifolia
Plant Mol. Biol.
76
475-488
2011
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2
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24
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24
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2
2
720280
Phogat
Sequence analysis, in silico m ...
Populus trichocarpa
J. Mol. Model.
16
1461-1471
2010
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703317
Riboulet
Kinetics of phenylpropanoid ge ...
Zea mays
Crop Sci.
49
211-223
2009
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705225
Sekhar Pagadala
Phylogenetic analysis, homolog ...
Leucaena leucocephala
J. Mol. Model.
15
203-221
2009
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2
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706277
Day
Caffeoyl coenzyme A O-methyltr ...
Linum usitatissimum
Plant Physiol. Biochem.
47
9-19
2009
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700855
Lee
Cation dependent O-methyltrans ...
Oryza sativa
Planta
227
641-647
2008
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1
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7
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1
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4
4
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1
702477
Ma
Characterization of a caffeic ...
Nicotiana tabacum, Triticum aestivum
Biochimie
90
515-524
2008
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2
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2
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1
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9
1
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2
2
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705354
Miao
-
Isolation of a novel tomato ca ...
Solanum lycopersicum, Solanum peruvianum
J. Phytopathol.
156
588-596
2008
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2
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2
1
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689675
Do
Both caffeoyl Coenzyme A 3-O-m ...
Arabidopsis thaliana
Planta
226
1117-1129
2007
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675991
Imin
Low temperature treatment at t ...
Oryza sativa
Mol. Cell. Proteomics
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