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Literature summary for 2.1.1.104 extracted from

  • Lukacin, R.; Matern, U.; Specker, S.; Vogt, T.
    Cations modulate the substrate specificity of bifunctional class I O-methyltransferase from Ammi majus (2004), FEBS Lett., 577, 367-370.
    View publication on PubMed
Search for more literature for 2.1.1.104

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ammi majus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.15 mM, completely abolishes the rate of methylation of caffeoyl-CoA Ammi majus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0041
-
 caffeoyl-CoA pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus
0.009
-
 quercetagetin pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus
0.02
-
 caffeoyl-CoA pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
0.031
-
 quercetin pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus
0.039
-
 3,4-dihydroxybenzoic acid pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
0.061
-
 caffeoyl-D-glucose pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
0.068
-
 5-hydroxyferulic acid pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
0.073
-
 quercetin pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
0.088
-
 caffeic acid pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
0.93
-
 caffeoyl-D-glucose pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.15 mM, about 2fold increase of the rate of methylation of caffeoyl-CoA. Replacing Co2+ for Mg2+ drastically shifts the substrate preferences towards caffeic, 5-hydroxyferulic and 3,4-dihydroxybenzoic acid, which are not at all accepted in assays with Mg2+ as cofactor Ammi majus
Mg2+ required Ammi majus
Mn2+ 0.15 mM, about 2fold increase of the rate of methylation of caffeoyl-CoA. Replacing Mn2+ for Mg2+ drastically shifts the substrate preferences towards caffeic, 5-hydroxyferulic and 3,4-dihydroxybenzoic acid, which are not at all accepted in assays with Mg2+ as cofactor Ammi majus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 x * 27000, SDS-PAGE Ammi majus

Organism

Organism UniProt Comment Textmining
Ammi majus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Ammi majus

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:dark-grown cell
-
 Ammi majus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 3,4-dihydroxybenzoic acid activity in presence of Co2+, no activity in presence of Mg2+ Ammi majus S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + 5-hydroxy-ferulic acid activity in presence of Co2+, no activity in presence of Mg2+ Ammi majus S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + caffeic acid activity in presence of Co2+, no activity in presence of Mg2+ Ammi majus S-adenosyl-L-homocysteine + ferulic acid
-
 ?
S-adenosyl-L-methionine + caffeoyl-CoA
-
 Ammi majus S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
S-adenosyl-L-methionine + caffeoyl-D-glucose
-
 Ammi majus S-adenosyl-L-homocysteine + feruloyl-D-glucose
-
 ?
S-adenosyl-L-methionine + quercetagetin no activity in presence of Co2+, low activity in presence of Mg2+ Ammi majus S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + quercetin
-
 Ammi majus S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 27000, SDS-PAGE Ammi majus

Synonyms

Synonyms Comment Organism
caffeoyl-coenzyme A O-methyltransferase
-
 Ammi majus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
 3,4-dihydroxybenzoic acid pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
0.068
-
 caffeic acid pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
0.071
-
 quercetagetin pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus
0.143
-
 5-hydroxyferulic acid pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
0.149
-
 caffeoyl-CoA pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus
0.206
-
 caffeoyl-D-glucose pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus
0.268
-
 quercetin pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus
0.631
-
 caffeoyl-D-glucose pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
0.938
-
 quercetin pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
1.043
-
 caffeoyl-CoA pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
6.08
-
 quercetin pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
6.08
-
 caffeoyl-CoA pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
6.08
-
 caffeoyl-D-glucose pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus