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Literature summary for 2.1.1.104 extracted from

  • Pakusch, A.E.; Matern, U.; Schiltz, E.
    Elicitor-inducible caffeoyl-coenzyme A 3-O-methyltransferase from Petroselinum crispum cell suspensions (1991), Plant Physiol., 95, 137-143.
    View publication on PubMedView publication on EuropePMC
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 2 * 24000, SDS-PAGE Petroselinum crispum
50000
-
 PAGE Petroselinum crispum

Organism

Organism UniProt Comment Textmining
Petroselinum crispum
-
 parsley
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on Blue Sepharose CL-6B, isoelectric focusing Petroselinum crispum

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture treated with crude cell wall elicitor from Phytophthora megasperma f.sp. glycinea Petroselinum crispum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0313
-
 unreliable, great loss during purification Petroselinum crispum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + caffeoyl-CoA
-
 Petroselinum crispum S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 24000, SDS-PAGE Petroselinum crispum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Petroselinum crispum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 unstable below Petroselinum crispum