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Literature summary for 2.1.1.101 extracted from
- Purification, characterization, and kinetic mechanism of S-adenosyl-L-methionine:macrocin O-methyltransferase from Streptomyces fradiae (1988), J. Biol. Chem., 263, 15619-15625.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | tylosin is used in the swine industry as a growth promotant | Streptomyces fradiae |
| pharmacology | tylosin is used in veterinary medicine for treatment of infections caused by gram-positive bacteria and mycoplasma | Streptomyces fradiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| demethyllactenocin | - |
Streptomyces fradiae |  |
| Demethylmacrocin | - |
Streptomyces fradiae | |
| Desmycosin | weak inhibition | Streptomyces fradiae | |
| Relomycin | weak inhibition | Streptomyces fradiae | |
| S-adenosyl-L-homocysteine | - |
Streptomyces fradiae | |
| sinefungin | competitive inhibition, potent inhibitor | Streptomyces fradiae | |
| tylosin | - |
Streptomyces fradiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can substitute Mg2+ | Streptomyces fradiae | |
| Mg2+ | required for maximal activity | Streptomyces fradiae | |
| Mn2+ | can substitute Mg2+ | Streptomyces fradiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000, SDS-PAGE | Streptomyces fradiae |
| 65000 | - |
gel filtration | Streptomyces fradiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + macrocin | Streptomyces fradiae | terminal and main rate-limiting step in tylosin biosynthesis | S-adenosylhomocysteine + tylosin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces fradiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces fradiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.061 | - |
- |
Streptomyces fradiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, quite stable in presence of 0.2 mM S-adenosyl-L-methionine and 10% ethanol, pH 7.0, stable over 6 months | Streptomyces fradiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | demethylmacrocin, demethyllactenocin, N5-methyltetrahydrofolate, betaine, choline, methylthioadenosine and methionine are not substrates | Streptomyces fradiae | ? | - |
? | |
| S-adenosyl-L-methionine + 20-dihydrolactenocin | - |
Streptomyces fradiae | S-adenosyl-L-homocysteine + ? | - |
? | |
| S-adenosyl-L-methionine + dihydromacrocin | - |
Streptomyces fradiae | S-adenosyl-L-homocysteine + ? | - |
? | |
| S-adenosyl-L-methionine + lactenocin | - |
Streptomyces fradiae | S-adenosyl-L-homocysteine + desmycosin | - |
? | |
| S-adenosyl-L-methionine + macrocin | - |
Streptomyces fradiae | S-adenosyl-L-homocysteine + tylosin | - |
? | |
| S-adenosyl-L-methionine + macrocin | terminal and main rate-limiting step in tylosin biosynthesis | Streptomyces fradiae | S-adenosylhomocysteine + tylosin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 32000, SDS-PAGE | Streptomyces fradiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 31 | - |
- |
Streptomyces fradiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Streptomyces fradiae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00006 | - |
sinefungin | - |
Streptomyces fradiae | |
| 0.00025 | - |
sinefungin | - |
Streptomyces fradiae | |
| 0.005 | - |
Demethylmacrocin | - |
Streptomyces fradiae | |
| 0.006 | - |
4'-demethyllactenocin | - |
Streptomyces fradiae | |
| 0.008 | - |
S-adenosyl-L-homocysteine | fixed substrate macrocin | Streptomyces fradiae | |
| 0.023 | - |
Desmycosin | fixed substrate S-adenosyl-L-methionine | Streptomyces fradiae | |
| 0.025 | - |
S-adenosyl-L-homocysteine | fixed substrate S-adenosyl-L-methionine | Streptomyces fradiae | |
| 0.026 | - |
Desmycosin | fixed substrate macrocin | Streptomyces fradiae | |
| 0.079 | - |
tylosin | fixed substrate S-adenosyl-L-methionine | Streptomyces fradiae | |
| 0.217 | - |
tylosin | fixed substrate lactenocin | Streptomyces fradiae | |
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