Cloned (Comment) | Organism |
---|---|
Icmt, expression in HEK-293 cells and Cos-1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D122A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
E107A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
E142A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
E146A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
E251A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
E252A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
F124A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
F124R | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
F258A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
F72A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
additional information | construction of various CFP-Icmt C-terminal truncation mutants, overview, comparison of membrane topology | Homo sapiens |
P275A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
R209A | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
R63A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
RH209/210A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
S123A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
W241A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
Y266A | site-directed mutagenesis, the mutant shows increased activity comapred to the wild-type enzyme, comparison of membrane topology | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | integral membrane protein with 6 transmembrane segments, determination and analysis of membrane topology using recombinant Icmt expressed in COS-1 cells, overview | Homo sapiens | 5789 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities of wild-type and mutant enzymes expressed in HEK-293 cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + N-biotinyl-S-farnesyl-L-cysteine peptide | - |
Homo sapiens | S-adenosyl-L-homocysteine + biotin S-farnesyl-L-cysteine peptide methyl ester | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Icmt | - |
Homo sapiens |
isoprenylcysteine carboxyl methyltransferase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Homo sapiens |