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Literature summary for 2.1.1.10 extracted from
- Biochemical and molecular characterization of the homocysteine S-methyltransferase from broccoli (Brassica oleracea var. italica) (2007), Phytochemistry, 68, 1112-1119.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Brassica oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-methionine | 0.5 mM, almost complete inhibition | Brassica oleracea |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.072 | - |
DL-homocysteine | - |
Brassica oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Brassica oleracea | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35400 | - |
calculated from amino acid sequence | Brassica oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica oleracea | A4ZGQ8 | var. italica, broccoli | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| plant | - |
Brassica oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0009 | - |
using L-selenocysteine as substrate | Brassica oleracea |
| 0.0017 | - |
using DL-selenocysteine as substrate | Brassica oleracea |
| 0.0054 | - |
using L-cysteine as substrate | Brassica oleracea |
| 0.0147 | - |
using DL-cysteine as substrate | Brassica oleracea |
| 0.0936 | - |
using DL-homocysteine as substrate | Brassica oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DL-cysteine | - |
Brassica oleracea | S-adenosyl-L-homocysteine + S-methyl-DL-cysteine | - |
? | |
| S-adenosyl-L-methionine + DL-homocysteine | - |
Brassica oleracea | L-methionine + S-adenosyl-L-homocysteine | - |
? | |
| S-adenosyl-L-methionine + DL-selenocysteine | - |
Brassica oleracea | S-adenosyl-L-homocysteine + Se-methyl-DL-selenocysteine | - |
? | |
| S-adenosyl-L-methionine + L-cysteine | - |
Brassica oleracea | S-adenosyl-L-homocysteine + S-methyl-L-cysteine | - |
? | |
| S-adenosyl-L-methionine + L-selenocysteine | - |
Brassica oleracea | S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HMT | - |
Brassica oleracea |
| S-methylmethionine: homocysteine S-methyltransferase | - |
Brassica oleracea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0375 | - |
DL-homocysteine | - |
Brassica oleracea | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Brassica oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Brassica oleracea |
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Release 2023.1 (January 2023)
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