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Literature summary for 2.1.1.1 extracted from
- HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples: evidence for substrate inhibition kinetics (2013), J. Chromatogr. B, 921-922, 87-95.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| imipramine | slightly but significantly activates the mouse liver NNMT enzyme and also changes the kinetic parameters Vmax, Km and Ki | Mus musculus |  |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | inhibition of NNMT activity is a promising therapeutic target for cancer therapy | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| nicotinamide | the enzyme shows substrate inhibition kinetics | Homo sapiens | |
| nicotinamide | the enzyme shows substrate inhibition kinetics | Mus musculus | |
| nicotinamide | the enzyme shows substrate inhibition kinetics | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Mus musculus | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
| additional information | - |
additional information | Michaelis-Menten kinetics, the Km value cannot be determined using Michaelis-Menten kinetic modelling | Oryctolagus cuniculus | |
| 1.02 | - |
nicotinamide | pH 7.2, 37°C, liver homogenate | Mus musculus | |
| 1.36 | - |
nicotinamide | pH 7.2, 37°C, liver homogenate | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mus musculus | development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview | ? | - |
? | |
| additional information | Homo sapiens | development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview | ? | - |
? | |
| additional information | Oryctolagus cuniculus | development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview | ? | - |
? | |
| S-adenosyl-L-methionine + nicotinamide | Mus musculus | - |
S-adenosyl-L-homocysteine + 1-methylnicotinamide | - |
? | |
| S-adenosyl-L-methionine + nicotinamide | Homo sapiens | - |
S-adenosyl-L-homocysteine + 1-methylnicotinamide | - |
? | |
| S-adenosyl-L-methionine + nicotinamide | Oryctolagus cuniculus | - |
S-adenosyl-L-homocysteine + 1-methylnicotinamide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview | Mus musculus | ? | - |
? | |
| additional information | development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview | Homo sapiens | ? | - |
? | |
| additional information | development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview | Oryctolagus cuniculus | ? | - |
? | |
| S-adenosyl-L-methionine + nicotinamide | - |
Mus musculus | S-adenosyl-L-homocysteine + 1-methylnicotinamide | - |
? | |
| S-adenosyl-L-methionine + nicotinamide | - |
Homo sapiens | S-adenosyl-L-homocysteine + 1-methylnicotinamide | - |
? | |
| S-adenosyl-L-methionine + nicotinamide | - |
Oryctolagus cuniculus | S-adenosyl-L-homocysteine + 1-methylnicotinamide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NNMT | - |
Mus musculus |
| NNMT | - |
Homo sapiens |
| NNMT | - |
Oryctolagus cuniculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Oryctolagus cuniculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.02 | - |
nicotinamide | pH 7.2, 37°C, liver homogenate | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Mus musculus |
| 7.2 | - |
assay at | Homo sapiens |
| 7.2 | - |
assay at | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Mus musculus | |
| S-adenosyl-L-methionine | - |
Homo sapiens | |
| S-adenosyl-L-methionine | - |
Oryctolagus cuniculus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.57 | - |
nicotinamide | pH 7.2, 37°C, liver homogenate | Oryctolagus cuniculus | |
| 20.01 | - |
nicotinamide | pH 7.2, 37°C, liver homogenate | Homo sapiens | |
| 50.64 | - |
nicotinamide | pH 7.2, 37°C, liver homogenate | Mus musculus | |
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