Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.1 extracted from

  • Tomida, M.; Ohtake, H.; Yokota, T.; Kobayashi, Y.; Kurosumi, M.
    Stat3 up-regulates expression of nicotinamide N-methyltransferase in human cancer cells (2008), J. Cancer Res. Clin. Oncol., 134, 551-559.
    View publication on PubMed

Application

Application Comment Organism
pharmacology molecular targets for cancer therapy and diagnosis Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, NNMT promoter region, promoter-luciferase reporter construct generated, pGL2 vector Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P40261
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, gel fitration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell no expression of NNMT in Homo sapiens
-
Hep-G2 cell NNMT expression increased on stimulation of the cells with interleukin 6, NNMT promoter activity dependent on the activation of Stat3 Homo sapiens
-
HT-29 cell colon cancer cells with high and constitutive expression of NNMT Homo sapiens
-
MCF-7 cell no expression of NNMT in Homo sapiens
-
MDA-MB-468 cell breast cancer cells with high and constitutive expression of NNMT Homo sapiens
-
SW-480 cell colon cancer cells, NNMT expression increased on stimulation of the cells with interleukin 6 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
signal transducers and activators of transcription 3 (Stat3)-regulated genes analyzed, expression of NNMT in cancer cells, promoter activity determined after transfection experiments with NNMT promoter-luciferase reporter construct, real-time PCR, Western-Blot and immunohistochemistry, correlation between the expression of NNMT and activated Stat3 in colon cancer cells, NNMT determined as a Stat3-regulated gene and as a potential tumor marker Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + nicotinamide NNMT as potential candidate for a tumor marker Homo sapiens S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
?

Synonyms

Synonyms Comment Organism
NNMT
-
Homo sapiens