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Literature summary for 1.97.1.9 extracted from

  • Mesbahi-Nowrouzi, M.; Mollania, N.
    Purification of selenate reductase from Alcaligenes sp. CKCr-6A with the ability to biosynthesis of selenium nanoparticle Enzymatic behavior study in imidazolium based ionic liquids and organic solvent (2018), J. Mol. Liq., 249, 1254-1262 .
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 70% inhibition at 1 mM Alcaligenes sp.
Ca2+ about 20% inhibition at 1 mM Alcaligenes sp.
EDTA inhibits the enzymatic reducing activity slightly, addition of Mo2+ and Zn2+ to the reaction mixture eliminates the EDTA inhibitory effect Alcaligenes sp.
Hg2+ complete inhibition at 1 mM Alcaligenes sp.
Mn2+ 35% inhibition at 1 mM Alcaligenes sp.
N-ethyl maleimide complete inhibition Alcaligenes sp.
NaN3 60% inhibition at 1 mM Alcaligenes sp.
Pb2+ 45% inhibition at 1 mM Alcaligenes sp.
Zn2+ 30% inhibition at 1 mM Alcaligenes sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
selenate pH 6.0, 35°C Alcaligenes sp.
5.47
-
Selenite pH 6.0, 35°C Alcaligenes sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Alcaligenes sp. 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mo2+ role of Mo2+ in selenate reductase Alcaligenes sp.
additional information Na+ and Mg2+ have no effect on the activity at 1 mM Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
197000
-
gel filtration Alcaligenes sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
selenate + NADH + H+ Alcaligenes sp.
-
selenite + H2O + NAD+
-
?
selenate + NADH + H+ Alcaligenes sp. CKCr-6A
-
selenite + H2O + NAD+
-
?
selenite + NADH + H+ Alcaligenes sp.
-
selenium + H2O + NAD+
-
?
selenite + NADH + H+ Alcaligenes sp. CKCr-6A
-
selenium + H2O + NAD+
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Methanol decreases enzyme activity but increases thermal inactivation of the enzyme Alcaligenes sp.
n-propanol decreases enzyme activity but increases thermal inactivation of the enzyme Alcaligenes sp.

Organism

Organism UniProt Comment Textmining
Alcaligenes sp.
-
isolated from Sabzevar of Iran
-
Alcaligenes sp. CKCr-6A
-
isolated from Sabzevar of Iran
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 21.29fold by ammonium sulfate fractionation, anion exchange and hydrophonic interaction chromatography, followed by ultrafiltration Alcaligenes sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.36
-
purified enzyme, pH 6.0, 35°C Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
selenate + NADH + H+
-
Alcaligenes sp. selenite + H2O + NAD+
-
?
selenate + NADH + H+
-
Alcaligenes sp. CKCr-6A selenite + H2O + NAD+
-
?
selenite + NADH + H+
-
Alcaligenes sp. selenium + H2O + NAD+
-
?
selenite + NADH + H+
-
Alcaligenes sp. CKCr-6A selenium + H2O + NAD+
-
?

Subunits

Subunits Comment Organism
heterotetramer 1 * 70000 + 1 * 45000 + 1 * 35000 + 1 *27000, SDS-PAGE Alcaligenes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Alcaligenes sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 activity range, profile overview, 70% and 35% of maximum activity at 50°C and 60°C Alcaligenes sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermal stability analysis by differential scanning calorim Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Alcaligenes sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9 activity range, profile overview Alcaligenes sp.

General Information

General Information Comment Organism
physiological function cell-free extracts of Alcaligenes sp. CKCr-6A catalyzes the NADH-dependent reduction of selenate and selenite to elemental selenium Alcaligenes sp.