Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 70% inhibition at 1 mM | Alcaligenes sp. | |
Ca2+ | about 20% inhibition at 1 mM | Alcaligenes sp. | |
EDTA | inhibits the enzymatic reducing activity slightly, addition of Mo2+ and Zn2+ to the reaction mixture eliminates the EDTA inhibitory effect | Alcaligenes sp. | |
Hg2+ | complete inhibition at 1 mM | Alcaligenes sp. | |
Mn2+ | 35% inhibition at 1 mM | Alcaligenes sp. | |
N-ethyl maleimide | complete inhibition | Alcaligenes sp. | |
NaN3 | 60% inhibition at 1 mM | Alcaligenes sp. | |
Pb2+ | 45% inhibition at 1 mM | Alcaligenes sp. | |
Zn2+ | 30% inhibition at 1 mM | Alcaligenes sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
selenate | pH 6.0, 35°C | Alcaligenes sp. | |
5.47 | - |
Selenite | pH 6.0, 35°C | Alcaligenes sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Alcaligenes sp. | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mo2+ | role of Mo2+ in selenate reductase | Alcaligenes sp. | |
additional information | Na+ and Mg2+ have no effect on the activity at 1 mM | Alcaligenes sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
197000 | - |
gel filtration | Alcaligenes sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
selenate + NADH + H+ | Alcaligenes sp. | - |
selenite + H2O + NAD+ | - |
? | |
selenate + NADH + H+ | Alcaligenes sp. CKCr-6A | - |
selenite + H2O + NAD+ | - |
? | |
selenite + NADH + H+ | Alcaligenes sp. | - |
selenium + H2O + NAD+ | - |
? | |
selenite + NADH + H+ | Alcaligenes sp. CKCr-6A | - |
selenium + H2O + NAD+ | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Methanol | decreases enzyme activity but increases thermal inactivation of the enzyme | Alcaligenes sp. |
n-propanol | decreases enzyme activity but increases thermal inactivation of the enzyme | Alcaligenes sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes sp. | - |
isolated from Sabzevar of Iran | - |
Alcaligenes sp. CKCr-6A | - |
isolated from Sabzevar of Iran | - |
Purification (Comment) | Organism |
---|---|
native enzyme 21.29fold by ammonium sulfate fractionation, anion exchange and hydrophonic interaction chromatography, followed by ultrafiltration | Alcaligenes sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.36 | - |
purified enzyme, pH 6.0, 35°C | Alcaligenes sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
selenate + NADH + H+ | - |
Alcaligenes sp. | selenite + H2O + NAD+ | - |
? | |
selenate + NADH + H+ | - |
Alcaligenes sp. CKCr-6A | selenite + H2O + NAD+ | - |
? | |
selenite + NADH + H+ | - |
Alcaligenes sp. | selenium + H2O + NAD+ | - |
? | |
selenite + NADH + H+ | - |
Alcaligenes sp. CKCr-6A | selenium + H2O + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 1 * 70000 + 1 * 45000 + 1 * 35000 + 1 *27000, SDS-PAGE | Alcaligenes sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Alcaligenes sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | activity range, profile overview, 70% and 35% of maximum activity at 50°C and 60°C | Alcaligenes sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal stability analysis by differential scanning calorim | Alcaligenes sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Alcaligenes sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | activity range, profile overview | Alcaligenes sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | cell-free extracts of Alcaligenes sp. CKCr-6A catalyzes the NADH-dependent reduction of selenate and selenite to elemental selenium | Alcaligenes sp. |