BRENDA - Enzyme Database
show all sequences of 1.97.1.9

Quantitative detection of selenate-reducing bacteria by real-time PCR targeting the selenate reductase gene

Wen, L.L.; Lai, C.Y.; Yang, Q.; Chen, J.X.; Zhang, Y.; Ontiveros-Valencia, A.; Zhao, H.P.; Enzyme Microb. Technol. 85, 19-24 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene serA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative PCR expression analysis
Thauera selenatis
gene serA, quantitative PCR expression analysis
Pseudomonas stutzeri
gene serA, quantitative PCR expression analysis
Thauera sp.
Engineering
Protein Variants
Commentary
Organism
additional information
development of a serA gene-based PCR primer set to target selenate reductase (SerA) for detecting selenate reducing bacteria (SeRB) in biofilms, the primer set is specific for selenate reducing bacteria but not denitrifying bacteria, overview. The lab cultured Pseudomonas stutzeri strain is used as positive control
Pseudomonas stutzeri
additional information
development of a serA gene-based PCR primer set to target selenate reductase (SerA) for detecting selenate reducing bacteria (SeRB) in biofilms, the primer set is specific for selenate reducing bacteria but not denitrifying bacteria, overview
Thauera selenatis
additional information
development of a serA gene-based PCR primer set to target selenate reductase (SerA) for detecting selenate reducing bacteria (SeRB) in biofilms, the primer set is specific for selenate reducing bacteria but not denitrifying bacteria, overview. The lab cultured Thauera sp. strain is used as positive control
Thauera sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
selenate + reduced acceptor
Pseudomonas stutzeri
-
selenite + H2O + acceptor
-
-
?
selenate + reduced acceptor
Thauera sp.
-
selenite + H2O + acceptor
-
-
?
selenate + reduced acceptor
Thauera selenatis
-
selenite + H2O + acceptor
-
-
?
selenate + reduced acceptor
Thauera selenatis ATCC 55363
-
selenite + H2O + acceptor
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas stutzeri
-
-
-
Thauera selenatis
Q9S1H0 and Q9S1G9 and Q9S1G7
alpha, beta, and gamma subunits serA, serB, and serC of selenate reductase; from selenate-reducing membrane biofilm reactor (MBfR) biofilms
-
Thauera selenatis ATCC 55363
Q9S1H0 and Q9S1G9 and Q9S1G7
alpha, beta, and gamma subunits serA, serB, and serC of selenate reductase; from selenate-reducing membrane biofilm reactor (MBfR) biofilms
-
Thauera sp.
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
selenate + reduced acceptor
-
744808
Pseudomonas stutzeri
selenite + H2O + acceptor
-
-
-
?
selenate + reduced acceptor
-
744808
Thauera sp.
selenite + H2O + acceptor
-
-
-
?
selenate + reduced acceptor
-
744808
Thauera selenatis
selenite + H2O + acceptor
-
-
-
?
selenate + reduced acceptor
-
744808
Thauera selenatis ATCC 55363
selenite + H2O + acceptor
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
selenate reductase
-
Pseudomonas stutzeri
selenate reductase
-
Thauera selenatis
selenate reductase
-
Thauera sp.
serA
-
Pseudomonas stutzeri
serA
-
Thauera selenatis
serA
-
Thauera sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
gene serA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative PCR expression analysis
Thauera selenatis
gene serA, quantitative PCR expression analysis
Pseudomonas stutzeri
gene serA, quantitative PCR expression analysis
Thauera sp.
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
development of a serA gene-based PCR primer set to target selenate reductase (SerA) for detecting selenate reducing bacteria (SeRB) in biofilms, the primer set is specific for selenate reducing bacteria but not denitrifying bacteria, overview. The lab cultured Pseudomonas stutzeri strain is used as positive control
Pseudomonas stutzeri
additional information
development of a serA gene-based PCR primer set to target selenate reductase (SerA) for detecting selenate reducing bacteria (SeRB) in biofilms, the primer set is specific for selenate reducing bacteria but not denitrifying bacteria, overview
Thauera selenatis
additional information
development of a serA gene-based PCR primer set to target selenate reductase (SerA) for detecting selenate reducing bacteria (SeRB) in biofilms, the primer set is specific for selenate reducing bacteria but not denitrifying bacteria, overview. The lab cultured Thauera sp. strain is used as positive control
Thauera sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
selenate + reduced acceptor
Pseudomonas stutzeri
-
selenite + H2O + acceptor
-
-
?
selenate + reduced acceptor
Thauera sp.
-
selenite + H2O + acceptor
-
-
?
selenate + reduced acceptor
Thauera selenatis
-
selenite + H2O + acceptor
-
-
?
selenate + reduced acceptor
Thauera selenatis ATCC 55363
-
selenite + H2O + acceptor
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
selenate + reduced acceptor
-
744808
Pseudomonas stutzeri
selenite + H2O + acceptor
-
-
-
?
selenate + reduced acceptor
-
744808
Thauera sp.
selenite + H2O + acceptor
-
-
-
?
selenate + reduced acceptor
-
744808
Thauera selenatis
selenite + H2O + acceptor
-
-
-
?
selenate + reduced acceptor
-
744808
Thauera selenatis ATCC 55363
selenite + H2O + acceptor
-
-
-
?
Other publictions for EC 1.97.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745578
Mesbahi-Nowrouzi
-
Purification of selenate redu ...
Alcaligenes sp., Alcaligenes sp. CKCr-6A
J. Mol. Liq.
249
1254-1262
2018
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2
1
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1
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2
2
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1
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1
1
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1
1
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9
-
2
1
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4
2
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1
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1
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4
1
1
1
1
-
1
1
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1
1
-
-
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744727
Subedi
Simultaneous selenate reducti ...
Clostridium sp., Clostridium ultunense, Exiguobacterium sp., Gottschalkia acidurici, Gottschalkia acidurici ATCC 7906, Lysinibacillus sp., Pseudomonas sp., Thauera sp. MZ1T, Tissierella sp.
Chemosphere
183
536-545
2017
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8
-
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8
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18
-
17
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18
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2
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8
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18
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18
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744808
Wen
Quantitative detection of sel ...
Pseudomonas stutzeri, Thauera selenatis, Thauera selenatis ATCC 55363, Thauera sp.
Enzyme Microb. Technol.
85
19-24
2016
-
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3
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3
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4
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7
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9
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4
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745727
Connelly
Biosynthesis of selenate redu ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2a
Microbiology
162
2136-2146
2016
-
-
1
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5
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1
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2
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6
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2
2
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1
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5
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2
2
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1
1
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744931
Yee
Selenate reductase activity i ...
Escherichia coli
FEMS Microbiol. Lett.
361
138-143
2014
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2
-
2
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2
1
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1
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1
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1
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2
1
1
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-
1
1
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-
727752
Kuroda
Molecular cloning and characte ...
Bacillus selenatarsenatis, Bacillus selenatarsenatis SF-1
J. Bacteriol.
193
2141-2148
2011
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-
1
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14
-
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3
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1
3
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714365
Dridge
Thermostable properties of the ...
Thauera selenatis
Biochimie
92
1268-1273
2010
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1
2
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1
3
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4
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1
1
2
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1
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1
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715508
Lowe
Quinol-cytochrome c oxidoreduc ...
Thauera selenatis
J. Biol. Chem.
285
18433-18442
2010
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1
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3
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1
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1
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697509
Hunter
Reduction of selenite to eleme ...
Pseudomonas sp., Pseudomonas sp. CA5
Curr. Microbiol.
58
493-498
2009
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-
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1
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2
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697647
Ma
Role of menaquinone biosynthes ...
Enterobacter cloacae, Enterobacter cloacae SLD1a-1 / ATCC 700258, Escherichia coli K-12
Environ. Microbiol.
11
149-158
2009
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12
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699361
Leaver
Enterobacter cloacae SLD1a-1 g ...
Enterobacter cloacae, Enterobacter cloacae SLD1a-1 / ATCC 700258
J. Ind. Microbiol. Biotechnol.
35
867-873
2008
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1
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2
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9
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2
1
1
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2
1
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1
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684986
Dridge
Investigation of the redox cen ...
Thauera selenatis
Biochem. J.
408
19-28
2007
-
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1
2
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3
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1
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671304
Ridley
Development of a viologen-base ...
Enterobacter cloacae, Enterobacter cloacae SLD1a-1 / ATCC 700258
Anal. Biochem.
358
289-294
2006
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2
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1
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7
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1
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671468
Ridley
Resolution of distinct membran ...
Enterobacter cloacae, Enterobacter cloacae SLD1a-1 / ATCC 700258
Appl. Environ. Microbiol.
72
5173-5180
2006
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2
1
2
3
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7
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1
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1
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657903
Watts
Microbial reduction of selenat ...
Enterobacter cloacae, Enterobacter cloacae SLDa-1, Sulfurospirillum barnesii, Thauera selenatis
Biochem. Soc. Trans.
33
173-175
2005
1
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658902
Maher
X-ray absorption spectroscopy ...
Thauera selenatis
Inorg. Chem.
43
402-404
2004
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2
4
1
1
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1
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644816
Watts
Selenate reduction by Enteroba ...
Enterobacter cloacae, Enterobacter cloacae SLD1a-1 / ATCC 700258
FEMS Microbiol. Lett.
228
273-279
2003
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2
2
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1
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11
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11
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644814
Maher
Crystallization and preliminar ...
Thauera selenatis
Acta Crystallogr. Sect. D
58
706-708
2002
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1
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1
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644815
Bebien
Involvement of a putative moly ...
Escherichia coli
Microbiology
148
3865-3872
2002
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2
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644813
Krafft
Cloning and sequencing of the ...
Thauera selenatis
DNA Seq.
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365-377
2000
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644812
Stolz
Bacterial respiration of arsen ...
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644811
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Purification and characterizat ...
Thauera selenatis
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1997
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644810
Rech
The terminal reductases for se ...
Thauera selenatis
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