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Literature summary for 1.97.1.9 extracted from

  • Maher, M.J.; Macy, J.M.
    Crystallization and preliminary X-ray analysis of the selenate reductase from Thauera selenatis (2002), Acta Crystallogr. Sect. D, 58, 706-708.
    View publication on PubMed
Search for more literature for 1.97.1.9

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion method, precipitation by ammonium sulfate, protein solution: 10 mg/ml, 0.3-0.5 M ammonium sulfate, 50 mM piperazine, pH 6.0, reservoir solution: 1.8-2.2 M ammonium sulfate, 100 mM Tris-HCl, pH 8.0-8.9, 293 K, 2-4 weeks, cryoprotection by 25% glycerol, X-ray structure determination and analysis Thauera selenatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 1 * 99000, alpha, + 1 * 37000, beta, + 1 * 23000, gamma, SDS-PAGE Thauera selenatis
37000
-
 1 * 99000, alpha, + 1 * 37000, beta, + 1 * 23000, gamma, SDS-PAGE Thauera selenatis
99000
-
 1 * 99000, alpha, + 1 * 37000, beta, + 1 * 23000, gamma, SDS-PAGE Thauera selenatis

Organism

Organism UniProt Comment Textmining
Thauera selenatis
-
 selenate-respiring bacterium
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thauera selenatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
selenate + electron donor
-
 Thauera selenatis selenite + H2O + oxidized electron donor
-
 ?

Subunits

Subunits Comment Organism
trimer 1 * 99000, alpha, + 1 * 37000, beta, + 1 * 23000, gamma, SDS-PAGE Thauera selenatis