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show all sequences of 1.97.1.2

Towards the reaction mechanism of pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici

Reichenbecher, W.; Schink, B.; Biochim. Biophys. Acta 1430, 245-253 (1999)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe
-
Pelobacter acidigallici
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pelobacter acidigallici
-
-
-
Oxidation Stability
Oxidation Stability
Organism
oxides replace the physiological cosubstrate, p.e. pyridine N-oxide, 2-hydroxypyridine N-oxide, DMSO and tetramethylene sulfoxide
Pelobacter acidigallici
Reaction
Reaction
Commentary
Organism
1,2,3,5-tetrahydroxybenzene + 1,2,3-trihydroxybenzene = 1,3,5-trihydroxybenzene + 1,2,3,5-tetrahydroxybenzene
a mechanism is proposed which combines an oxidative hydroxylation with a reductive dehydroxylation via molybdenum cofactor
Pelobacter acidigallici
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
oxides tested as cosubstrates
Pelobacter acidigallici
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2,3,5-tetrahydroxybenzene + hydroxyhydroquinone
-
437679
Pelobacter acidigallici
phloroglucinol + 1,2,4,5-tetrahydroxybenzene
-
437679
Pelobacter acidigallici
?
1,2,3,5-tetrahydroxybenzene + pyrogallol
pyrogallol i.e. 1,2,3-tri-hydroxybenzene
437679
Pelobacter acidigallici
phloroglucinol + 1,2,3,5-tetrahydroxybenzene
phloroglucinol i.e. 1,3,5-tri-hydroxybenzene
437679
Pelobacter acidigallici
?
additional information
pyrogallol converts to phloroglucinol in the absence of added tetrahydroxybenzene if very little pyrogallol and high amounts of enzyme are present
437679
Pelobacter acidigallici
?
-
-
-
-
additional information
water is not the source of the added hydroxyl groups
437679
Pelobacter acidigallici
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
molybdenum cofactor
-
Pelobacter acidigallici
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
molybdenum cofactor
-
Pelobacter acidigallici
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe
-
Pelobacter acidigallici
Oxidation Stability (protein specific)
Oxidation Stability
Organism
oxides replace the physiological cosubstrate, p.e. pyridine N-oxide, 2-hydroxypyridine N-oxide, DMSO and tetramethylene sulfoxide
Pelobacter acidigallici
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
oxides tested as cosubstrates
Pelobacter acidigallici
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2,3,5-tetrahydroxybenzene + hydroxyhydroquinone
-
437679
Pelobacter acidigallici
phloroglucinol + 1,2,4,5-tetrahydroxybenzene
-
437679
Pelobacter acidigallici
?
1,2,3,5-tetrahydroxybenzene + pyrogallol
pyrogallol i.e. 1,2,3-tri-hydroxybenzene
437679
Pelobacter acidigallici
phloroglucinol + 1,2,3,5-tetrahydroxybenzene
phloroglucinol i.e. 1,3,5-tri-hydroxybenzene
437679
Pelobacter acidigallici
?
additional information
pyrogallol converts to phloroglucinol in the absence of added tetrahydroxybenzene if very little pyrogallol and high amounts of enzyme are present
437679
Pelobacter acidigallici
?
-
-
-
-
additional information
water is not the source of the added hydroxyl groups
437679
Pelobacter acidigallici
?
-
-
-
-
Other publictions for EC 1.97.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
673091
Paizs
Investigation of the mechanism ...
Pelobacter acidigallici
Chemistry
13
2805-2811
2007
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1
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660360
Messerschmidt
Crystal structure of pyrogallo ...
Pelobacter acidigallici
Proc. Natl. Acad. Sci. USA
101
11571-11576
2004
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1
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2
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5
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1
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437680
Abt
Crystallization and preliminar ...
Pelobacter acidigallici, Pelobacter acidigallici MaGal 2
Acta Crystallogr. Sect. D
58
343-345
2002
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1
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2
1
2
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5
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1
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1
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6
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1
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1
2
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1
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1
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6
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437677
Baas
Cloning, sequencing and hetero ...
Pelobacter acidigallici
Eur. J. Biochem.
265
896-901
1999
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1
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1
3
1
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2
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3
1
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1
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1
1
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1
3
1
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3
1
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437679
Reichenbecher
Towards the reaction mechanism ...
Pelobacter acidigallici
Biochim. Biophys. Acta
1430
245-253
1999
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1
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3
1
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1
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1
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4
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1
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1
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1
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1
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1
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4
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437678
Reichenbecher
One molecule of molybdopterin ...
Pelobacter acidigallici
Eur. J. Biochem.
237
406-413
1996
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2
3
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3
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1
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2
1
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1
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1
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2
3
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2
1
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4734
Brune
-
Phloroglucinol pathway in the ...
Pelobacter acidigallici, Pelobacter acidigallici MaGal 2
Arch. Microbiol.
157
417-424
1992
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2
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4
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1
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6
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2
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1
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6
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437676
Brune
Sequential transhydroxylations ...
Pelobacter massiliensis
Appl. Environ. Microbiol.
58
1861-1868
1992
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1
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3
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11
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1
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1
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3
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11
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1
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1
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437674
Brune
Pyrogallol-to-phloroglucinol c ...
Pelobacter acidigallici, Pelobacter acidigallici MaGal 2
J. Bacteriol.
172
1070-1076
1990
1
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2
1
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1
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4
1
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1
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14
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1
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1
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1
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2
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1
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1
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1
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1
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14
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1
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1
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