BRENDA - Enzyme Database show
show all sequences of 1.97.1.2

Pyrogallol-to-phloroglucinol conversion and other hydroxyl-transfer reactions catalyzed by cell extracts of Pelobacter acidigallici

Brune, A.; Schink, B.; J. Bacteriol. 172, 1070-1076 (1990)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
no activation by reducing agents
Pelobacter acidigallici
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
oxygen does not inactivate, but the assay requires strict anaerobiosis due to the instability of the substrates and products
Pelobacter acidigallici
resorcinol
complete inhibition of the reaction of hydroxyhydroquinone with itself
Pelobacter acidigallici
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.71
-
1,2,3,5-Tetrahydroxybenzene
-
Pelobacter acidigallici
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1,2,3,5-tetrahydroxybenzene + pyrogallol
Pelobacter acidigallici
-
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pelobacter acidigallici
-
-
-
Pelobacter acidigallici MaGal 2
-
-
-
Oxidation Stability
Oxidation Stability
Organism
oxygen does not inactivate, but the assay requires strict anaerobiosis because of the instability of the substrates and products
Pelobacter acidigallici
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.23
-
dimethyl sulfoxide
Pelobacter acidigallici
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2,3,5-tetrahydroxybenzene + 1,2,3,5-tetrahydroxybenzene
-
437674
Pelobacter acidigallici
phloroglucinol + pentahydroxybenzene
tentatively identified
437674
Pelobacter acidigallici
?
1,2,3,5-tetrahydroxybenzene + 1,2,3,5-tetrahydroxybenzene
-
437674
Pelobacter acidigallici MaGal 2
phloroglucinol + pentahydroxybenzene
tentatively identified
437674
Pelobacter acidigallici MaGal 2
?
1,2,3,5-tetrahydroxybenzene + hydroxyhydroquinone
-
437674
Pelobacter acidigallici
phloroglucinol + 1,2,4,5-tetrahydroxybenzene
tentatively identified
437674
Pelobacter acidigallici
?
1,2,3,5-tetrahydroxybenzene + pyrogallol
pyrogallol i.e. 1,2,3-tri-hydroxybenzene
437674
Pelobacter acidigallici
phloroglucinol + 1,2,3,5-tetrahydroxybenzene
phloroglucinol i.e. 1,3,5-tri-hydroxybenzene
437674
Pelobacter acidigallici
r
1,2,3,5-tetrahydroxybenzene + pyrogallol
pyrogallol i.e. 1,2,3-tri-hydroxybenzene
437674
Pelobacter acidigallici MaGal 2
phloroglucinol + 1,2,3,5-tetrahydroxybenzene
phloroglucinol i.e. 1,3,5-tri-hydroxybenzene
437674
Pelobacter acidigallici MaGal 2
r
1,2,3,5-tetrahydroxybenzene + pyrogallol
-
437674
Pelobacter acidigallici
?
-
-
-
?
1,2,3,5-tetrahydroxybenzene + resorcinol
-
437674
Pelobacter acidigallici
phloroglucinol + hydroxyhydroquinone
-
437674
Pelobacter acidigallici
?
hydroxyhydroquinone + hydroxyhydroquinone
-
437674
Pelobacter acidigallici
resorcinol + 1,2,4,5-tetrahydroxybenzene
tentatively identified
437674
Pelobacter acidigallici
-
hydroxyhydroquinone + pyrogallol
hydroxyhydroquinone i.e. 1,2,4-trihydroxybenzene
437674
Pelobacter acidigallici
resorcinol + 1,2,3,5-tetrahydroxybenzene
resorcinol i.e. 1,3-benzenediol
437674
Pelobacter acidigallici
?
hydroxyhydroquinone + pyrogallol
hydroxyhydroquinone i.e. 1,2,4-trihydroxybenzene
437674
Pelobacter acidigallici MaGal 2
resorcinol + 1,2,3,5-tetrahydroxybenzene
resorcinol i.e. 1,3-benzenediol
437674
Pelobacter acidigallici MaGal 2
?
additional information
dimethyl sulfoxide at unphysiological high concentrations can replace 1,2,3,5-tetrahydroxybenzene with 0.04% efficiency, no transhydroxylation takes place with catechol or phenol + 1,2,3,5-tetrahydroxybenzene, pyrogallol + catechol, resorcinol, hydroquinone or phenol
437674
Pelobacter acidigallici
?
-
-
-
-
additional information
1,2,3,5-tetrahydroxybenzene, 1,2,4-trihydroxybenzene, effective OH-donors
437674
Pelobacter acidigallici
?
-
-
-
-
additional information
dimethyl sulfoxide at unphysiological high concentrations can replace 1,2,3,5-tetrahydroxybenzene with 0.04% efficiency, no transhydroxylation takes place with catechol or phenol + 1,2,3,5-tetrahydroxybenzene, pyrogallol + catechol, resorcinol, hydroquinone or phenol
437674
Pelobacter acidigallici MaGal 2
?
-
-
-
-
additional information
1,2,3,5-tetrahydroxybenzene, 1,2,4-trihydroxybenzene, effective OH-donors
437674
Pelobacter acidigallici MaGal 2
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Pelobacter acidigallici
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Pelobacter acidigallici
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
no activation by reducing agents
Pelobacter acidigallici
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
oxygen does not inactivate, but the assay requires strict anaerobiosis due to the instability of the substrates and products
Pelobacter acidigallici
resorcinol
complete inhibition of the reaction of hydroxyhydroquinone with itself
Pelobacter acidigallici
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.71
-
1,2,3,5-Tetrahydroxybenzene
-
Pelobacter acidigallici
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1,2,3,5-tetrahydroxybenzene + pyrogallol
Pelobacter acidigallici
-
?
-
-
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
oxygen does not inactivate, but the assay requires strict anaerobiosis because of the instability of the substrates and products
Pelobacter acidigallici
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.23
-
dimethyl sulfoxide
Pelobacter acidigallici
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2,3,5-tetrahydroxybenzene + 1,2,3,5-tetrahydroxybenzene
-
437674
Pelobacter acidigallici
phloroglucinol + pentahydroxybenzene
tentatively identified
437674
Pelobacter acidigallici
?
1,2,3,5-tetrahydroxybenzene + 1,2,3,5-tetrahydroxybenzene
-
437674
Pelobacter acidigallici MaGal 2
phloroglucinol + pentahydroxybenzene
tentatively identified
437674
Pelobacter acidigallici MaGal 2
?
1,2,3,5-tetrahydroxybenzene + hydroxyhydroquinone
-
437674
Pelobacter acidigallici
phloroglucinol + 1,2,4,5-tetrahydroxybenzene
tentatively identified
437674
Pelobacter acidigallici
?
1,2,3,5-tetrahydroxybenzene + pyrogallol
pyrogallol i.e. 1,2,3-tri-hydroxybenzene
437674
Pelobacter acidigallici
phloroglucinol + 1,2,3,5-tetrahydroxybenzene
phloroglucinol i.e. 1,3,5-tri-hydroxybenzene
437674
Pelobacter acidigallici
r
1,2,3,5-tetrahydroxybenzene + pyrogallol
pyrogallol i.e. 1,2,3-tri-hydroxybenzene
437674
Pelobacter acidigallici MaGal 2
phloroglucinol + 1,2,3,5-tetrahydroxybenzene
phloroglucinol i.e. 1,3,5-tri-hydroxybenzene
437674
Pelobacter acidigallici MaGal 2
r
1,2,3,5-tetrahydroxybenzene + pyrogallol
-
437674
Pelobacter acidigallici
?
-
-
-
?
1,2,3,5-tetrahydroxybenzene + resorcinol
-
437674
Pelobacter acidigallici
phloroglucinol + hydroxyhydroquinone
-
437674
Pelobacter acidigallici
?
hydroxyhydroquinone + hydroxyhydroquinone
-
437674
Pelobacter acidigallici
resorcinol + 1,2,4,5-tetrahydroxybenzene
tentatively identified
437674
Pelobacter acidigallici
-
hydroxyhydroquinone + pyrogallol
hydroxyhydroquinone i.e. 1,2,4-trihydroxybenzene
437674
Pelobacter acidigallici
resorcinol + 1,2,3,5-tetrahydroxybenzene
resorcinol i.e. 1,3-benzenediol
437674
Pelobacter acidigallici
?
hydroxyhydroquinone + pyrogallol
hydroxyhydroquinone i.e. 1,2,4-trihydroxybenzene
437674
Pelobacter acidigallici MaGal 2
resorcinol + 1,2,3,5-tetrahydroxybenzene
resorcinol i.e. 1,3-benzenediol
437674
Pelobacter acidigallici MaGal 2
?
additional information
dimethyl sulfoxide at unphysiological high concentrations can replace 1,2,3,5-tetrahydroxybenzene with 0.04% efficiency, no transhydroxylation takes place with catechol or phenol + 1,2,3,5-tetrahydroxybenzene, pyrogallol + catechol, resorcinol, hydroquinone or phenol
437674
Pelobacter acidigallici
?
-
-
-
-
additional information
1,2,3,5-tetrahydroxybenzene, 1,2,4-trihydroxybenzene, effective OH-donors
437674
Pelobacter acidigallici
?
-
-
-
-
additional information
dimethyl sulfoxide at unphysiological high concentrations can replace 1,2,3,5-tetrahydroxybenzene with 0.04% efficiency, no transhydroxylation takes place with catechol or phenol + 1,2,3,5-tetrahydroxybenzene, pyrogallol + catechol, resorcinol, hydroquinone or phenol
437674
Pelobacter acidigallici MaGal 2
?
-
-
-
-
additional information
1,2,3,5-tetrahydroxybenzene, 1,2,4-trihydroxybenzene, effective OH-donors
437674
Pelobacter acidigallici MaGal 2
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Pelobacter acidigallici
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Pelobacter acidigallici
Other publictions for EC 1.97.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
673091
Paizs
Investigation of the mechanism ...
Pelobacter acidigallici
Chemistry
13
2805-2811
2007
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1
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660360
Messerschmidt
Crystal structure of pyrogallo ...
Pelobacter acidigallici
Proc. Natl. Acad. Sci. USA
101
11571-11576
2004
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1
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437680
Abt
Crystallization and preliminar ...
Pelobacter acidigallici, Pelobacter acidigallici MaGal 2
Acta Crystallogr. Sect. D
58
343-345
2002
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1
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1
2
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1
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1
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6
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437677
Baas
Cloning, sequencing and hetero ...
Pelobacter acidigallici
Eur. J. Biochem.
265
896-901
1999
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1
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1
3
1
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2
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3
1
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1
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1
1
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3
1
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1
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437679
Reichenbecher
Towards the reaction mechanism ...
Pelobacter acidigallici
Biochim. Biophys. Acta
1430
245-253
1999
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1
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4
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437678
Reichenbecher
One molecule of molybdopterin ...
Pelobacter acidigallici
Eur. J. Biochem.
237
406-413
1996
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1
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3
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1
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4734
Brune
-
Phloroglucinol pathway in the ...
Pelobacter acidigallici, Pelobacter acidigallici MaGal 2
Arch. Microbiol.
157
417-424
1992
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437676
Brune
Sequential transhydroxylations ...
Pelobacter massiliensis
Appl. Environ. Microbiol.
58
1861-1868
1992
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437674
Brune
Pyrogallol-to-phloroglucinol c ...
Pelobacter acidigallici, Pelobacter acidigallici MaGal 2
J. Bacteriol.
172
1070-1076
1990
1
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14
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