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Literature summary for 1.97.1.12 extracted from

  • Kozuleva, M.; Vetoshkina, D.; Petrova, A.; Borisova-Mubarakshina, M.; Ivanov, B.
    The study of oxygen reduction in photosystem I of higher plants using electron donors for this photosystem in intact thylakoids (2015), Biochemistry (Moscow), 9, 246-251 .
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
3-(3,4-dichlorophenyl)-1,1-dimethylurea DCMU Pisum sativum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
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Pisum sativum 9507
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thylakoid
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Pisum sativum 9579
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Organism

Organism UniProt Comment Textmining
Pisum sativum
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var. Bogatyr
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information oxygen uptake in the light is analyzed in suspensions of isolated pea thylakoids upon inhibition of electron transport from photosystem II (PS II) by diuron and delivery of electrons to photosystem I (PS I) by means of artificial donors in the presence of ascorbate, O2 reduction in PS I. 2,6-dichlorophenolindophenol (DCPIP) cannot be used as the donor for photosystem I. N,N,N',N'-tetramethyl-p-phenylene diamine (TMPD), applied as a donor, does not affect immediately the reaction of the O2 reduction, since an increase in its concentration does not lead to an increase in the oxygen uptake rate in the light. In the experiments with TMPD, an increase in light intensity leads to an increase in the oxygen uptake rate, and this fact was interpreted as a consequence of the increase in the apparent rate constant of the reaction of the O2 reduction by the components of the acceptor side of photosystem I. TMPD redox transformation consists of only one step, while the DCPIP transformation includes two steps. Ascorbate is capable of donating electrons to the primary pair of PS I cofactors, P700+. Addition of either DCPIP or TMPD at concentration of 0.05 mM to the suspension containing both DCMU and ascorbate results in the twofold increase in the rate of the O2 consumption. O2 reduction by PS I uses ascorbate alone or in combination with lipophilic compounds as immediate donor of electrons Pisum sativum ?
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Synonyms

Synonyms Comment Organism
PS I
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Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
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assay at Pisum sativum