Literature summary for 1.9.6.1 extracted from

Mintmier, B.; McGarry, J.M.; Sparacino-Watkins, C.E.; Sallmen, J.; Fischer-Schrader, K.; Magalon, A.; McCormick, J.R.; Stolz, J.F.; Schwarz, G.; Bain, D.J.; Basu, P.
Molecular cloning, expression and biochemical characterization of periplasmic nitrate reductase from Campylobacter jejuni (2018), FEMS Microbiol. Lett., 365, fny151 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
homology modeling of structure, the substrate channel and catalytic pocket are more basic than other NapA proteins, which may influence the substrate binding as well as product release	Campylobacter jejuni

Protein Variants

Protein Variants	Comment	Organism
C176S	mutation in strictly conserved residue, significantly lower activity than wild-type	Campylobacter jejuni

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0034	-	nitrate	wild-type, pH 7.0, 25°C	Campylobacter jejuni
0.307	-	nitrate	mutant C176S, pH 7.0, 25°C	Campylobacter jejuni

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
periplasm	-	Campylobacter jejuni	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	Fe:Mo ratio is slightly higher than the theoretical value of 4:1	Campylobacter jejuni
Molybdenum	92% Mo incorporation in active CNapA. The Fe:Mo ratio is slightly higher than the theoretical value of 4:1	Campylobacter jejuni

Organism

Organism	UniProt	Comment	Textmining
Campylobacter jejuni	Q5HV12	-	-
Campylobacter jejuni RM1221	Q5HV12	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nitrate + reduced methyl viologen	-	Campylobacter jejuni	nitrite + oxidized methyl viologen + H2O	-	?
nitrate + reduced methyl viologen	-	Campylobacter jejuni RM1221	nitrite + oxidized methyl viologen + H2O	-	?

Synonyms

Synonyms	Comment	Organism
NapA	-	Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.045	-	nitrate	mutant C176S, pH 7.0, 25°C	Campylobacter jejuni
5.91	-	nitrate	wild-type, pH 7.0, 25°C	Campylobacter jejuni

Cofactor

Cofactor	Comment	Organism	Structure
[4Fe-4S]-center	-	Campylobacter jejuni

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1740	-	nitrate	wild-type, pH 7.0, 25°C	Campylobacter jejuni

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Release 2023.1 (January 2023)