Cloned (Comment) | Organism |
---|---|
cloning of napABC, quantitative PCR-based expression analysis | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | constructin of a napA mutant strain CAL277, and of several other nap gene mutants, overview | Salmonella enterica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Salmonella enterica | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferrocytochrome + 2 H+ + nitrate | Salmonella enterica | - |
2 ferricytochrome + nitrite | - |
r | |
2 ferrocytochrome + 2 H+ + nitrate | Salmonella enterica SL1344 AND CAL128 | - |
2 ferricytochrome + nitrite | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
serovar typhimurium | - |
Salmonella enterica SL1344 AND CAL128 | - |
serovar typhimurium | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferrocytochrome + 2 H+ + nitrate | - |
Salmonella enterica | 2 ferricytochrome + nitrite | - |
r | |
2 ferrocytochrome + 2 H+ + nitrate | - |
Salmonella enterica SL1344 AND CAL128 | 2 ferricytochrome + nitrite | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NapABC | - |
Salmonella enterica |
periplasmic nitrate reductase | - |
Salmonella enterica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Salmonella enterica |
General Information | Comment | Organism |
---|---|---|
malfunction | Salmonella enterica serovar Typhimurium strains with defects in either nitrate reductase A (narG mutant) or the regulator inducing its transcription in the presence of high concentrations of nitrate (narL mutant) exhibit growth comparable to that of wild-type Salmonella enterica serovar Typhimurium. In contrast, a strain lacking a functional periplasmic nitrate reductase (napA mutant) exhibits a marked growth defect in the lumen of the colon. Inactivation of narP, encoding a response regulator that activates napABC transcription in response to low nitrate concentrations, significantly reduces the growth of Salmonella enterica serovar Typhimurium in the murine host gut lumen | Salmonella enterica |
additional information | the Salmonella enterica serovar Typhimurium genome contains three nitrate reductases, encoded by the narGHI, narZYV, and napABC genes | Salmonella enterica |
physiological function | Salmonella enterica serovar Typhimurium uses the periplasmic nitrate reductase to support its growth on the low nitrate concentrations encountered in the gut, a strategy that may be shared with other enteric pathogens | Salmonella enterica |