Literature summary for 1.8.99.2 extracted from

Kim, S.K.; Rahman, A.; Conover, R.C.; Johnson, M.K.; Mason, J.T.; Gomes, V.; Hirasawa, M.; Moore, M.L.; Leustek, T.; Knaff, D.B.
Properties of the cysteine residues and the iron-sulfur cluster of the assimilatory 5-adenylyl sulfate reductase from Enteromorpha intestinalis (2006), Biochemistry, 45, 5010-5018.

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Activating Compound

Activating Compound	Comment	Organism	Structure
Na2SO4	100 mM	Ulva intestinalis

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Ulva intestinalis

Protein Variants

Protein Variants	Comment	Organism
C165S	reduced activity compared to wild type enzyme	Ulva intestinalis
C166S	reduced activity compared to wild type enzyme	Ulva intestinalis
C250S	reduced activity compared to wild type enzyme	Ulva intestinalis
C257S	reduced activity compared to wild type enzyme	Ulva intestinalis
C260S	reduced activity compared to wild type enzyme	Ulva intestinalis
C285S	reduced activity compared to wild type enzyme	Ulva intestinalis
C342S	reduced activity compared to wild type enzyme	Ulva intestinalis
C345S	reduced activity compared to wild type enzyme	Ulva intestinalis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	non-reducing and reducing SDS-PAGE	Ulva intestinalis
96000	-	non-reducing SDS-PAGE, 16% of the protein was present as a homodimer	Ulva intestinalis

Organism

Organism	UniProt	Comment	Textmining
Ulva intestinalis	O81350	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+ affinity chromatography	Ulva intestinalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.011	-	mutant enzyme 342S	Ulva intestinalis
0.15	-	mutant enzyme 345S	Ulva intestinalis
3.5	-	mutant enzyme C250S	Ulva intestinalis
7.248	-	wild type enzyme	Ulva intestinalis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5'-adenylylsulfate + dithiothreitol	-	Ulva intestinalis	adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol	-	ir

Subunits

Subunits	Comment	Organism
homodimer	2 * 48000, non-reducing SDS-PAGE, 16% of the protein is present as a homodimer	Ulva intestinalis
monomer	1 * 48000, non-reducing and reducing SDS-PAGE	Ulva intestinalis

Synonyms

Synonyms	Comment	Organism
5'-adenylyl sulfate reductase	-	Ulva intestinalis
EiAPR	-	Ulva intestinalis

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Release 2023.1 (January 2023)