Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli of a tagged recombinant protein | Bacillus subtilis |
General Stability | Organism |
---|---|
unstable to air with a half-life of 12 min, the 4Fe-4S cluster is converted into a 2Fe-2S form | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
reduced glutaredoxin 1 | pH 8 and saturating adenylyl sulfate | Bacillus subtilis | |
0.029 | - |
adenylyl sulfate | pH 8 and saturating thioredoxin 1 | Bacillus subtilis | |
0.053 | - |
thioredoxin 1 | pH 8 and saturating adenylyl sulfate | Bacillus subtilis | |
0.105 | - |
adenylyl sulfate | pH 8 and saturating glutaredoxin 1 | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains a cubane 4Fe-4S cluster | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
2 * 27000, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxygen causes complete loss of activity | Bacillus subtilis |
Purification (Comment) | Organism |
---|---|
purification of a recombinant tagged protein by chitin affinity chromatography | Bacillus subtilis |
Renatured (Comment) | Organism |
---|---|
the inactive 2Fe-2S form can be converted into the 4Fe-4S form by adding an iron-sulfur cluster synthesizing-protein from Synechocystis, restoring the activity | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenylyl sulfate + reduced glutaredoxin 1 | can use 3'-phosphoadenylyl sulfate as sulfate donor | Bacillus subtilis | AMP + sulfite + oxidized glutaredoxin 1 | - |
? | |
adenylyl sulfate + reduced thioredoxin 1 | can use 3'-phosphoadenylyl sulfate as sulfate donor | Bacillus subtilis | AMP + sulfite + oxidized thioredoxin 1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 27000, SDS-PAGE | Bacillus subtilis |