Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 10 mM 2% loss of the activity with ferricyanide, 19% loss of the activity with cytochrome c | Thiocapsa roseopersicina | |
NaN3 | 10 mM, no loss of the activity with ferricyanide, 52% loss of the activity with cytochrome c | Thiocapsa roseopersicina | |
NEM | 10 mM, 45% loss of activity with ferricyanide, complete loss of activity with cytochrome c | Thiocapsa roseopersicina | |
PCMB | 1 mM, 23% loss of the activity with ferricyanide, complete loss of activity with cytochrome c | Thiocapsa roseopersicina | |
sodium arsenite | 10 mM, no loss of the activity with ferricyanide, 88% loss of the activity with cytochrome c | Thiocapsa roseopersicina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
cytochrome c | - |
Thiocapsa roseopersicina | |
0.05 | - |
AMP | reaction with cytochrome c | Thiocapsa roseopersicina | |
0.073 | - |
sulfite | reaction with ferricyanide | Thiocapsa roseopersicina | |
0.093 | - |
sulfite | reaction with cytochrome c | Thiocapsa roseopersicina | |
0.13 | - |
ferricyanide | - |
Thiocapsa roseopersicina | |
1.5 | - |
sulfite | reaction with ferricyanide | Thiocapsa roseopersicina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains 4 mol of non-heme iron per mol of enzyme | Thiocapsa roseopersicina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thiocapsa roseopersicina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thiocapsa roseopersicina |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thiocapsa roseopersicina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + sulfite + cytochrome c | - |
Thiocapsa roseopersicina | adenylyl sulfate + ? | - |
? | |
AMP + sulfite + ferricyanide | - |
Thiocapsa roseopersicina | adenylyl sulfate + ferrocyanide | - |
? | |
CMP + sulfite + cytochrome c | 7% of the activity with AMP | Thiocapsa roseopersicina | cytidylyl sulfate + ? | - |
? | |
CMP + sulfite + ferricyanide | 8% of the activity with AMP | Thiocapsa roseopersicina | cytidylyl sulfate + ferrocyanide | - |
? | |
dAMP + sulfite + cytochrome c | 80% of the activity with AMP | Thiocapsa roseopersicina | deoxyadenylyl sulfate + ? | - |
? | |
deoxyAMP + sulfite + ferricyanide | 128% of maximal activity | Thiocapsa roseopersicina | deoxyadenylyl sulfate + ferrocyanide | - |
? | |
GMP + sulfite + cytochrome c | 9% of the activity with AMP | Thiocapsa roseopersicina | guanylyl sulfate + ? | - |
? | |
GMP + sulfite + ferricyanide | 51% of the activity with AMP | Thiocapsa roseopersicina | guanylyl sulfate + ferrocyanide | - |
? | |
IMP + sulfite + cytochrome c | 62% of the activity with AMP | Thiocapsa roseopersicina | ? | - |
? | |
IMP + sulfite + ferricyanide | 90% of the activity with AMP | Thiocapsa roseopersicina | ? + ferrocyanide | - |
? | |
UMP + sulfite + cytochrome c | 9% of the activity with AMP | Thiocapsa roseopersicina | uridylyl sulfate + ? | - |
? | |
UMP + sulfite + ferricyanide | 8% of the activity with AMP | Thiocapsa roseopersicina | uridylyl sulfate + ferrocyanide | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reaction with ferricyanide | Thiocapsa roseopersicina |
9 | - |
reaction with cytochrome c | Thiocapsa roseopersicina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme contains 1 mol of FAD per mol of enzyme | Thiocapsa roseopersicina | |
heme | contains 2 heme groups of cytochrome c characterper mol of enzyme | Thiocapsa roseopersicina |