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Literature summary for 1.8.99.2 extracted from

  • Trueper, H.G.; Rogers, L.A.
    Purification and properties of adenylyl sulfate reductase from phototrophic sulfur bacterium, Thiocapsa roseopersicina (1971), J. Bacteriol., 108, 1112-1121.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide 10 mM 2% loss of the activity with ferricyanide, 19% loss of the activity with cytochrome c Thiocapsa roseopersicina
NaN3 10 mM, no loss of the activity with ferricyanide, 52% loss of the activity with cytochrome c Thiocapsa roseopersicina
NEM 10 mM, 45% loss of activity with ferricyanide, complete loss of activity with cytochrome c Thiocapsa roseopersicina
PCMB 1 mM, 23% loss of the activity with ferricyanide, complete loss of activity with cytochrome c Thiocapsa roseopersicina
sodium arsenite 10 mM, no loss of the activity with ferricyanide, 88% loss of the activity with cytochrome c Thiocapsa roseopersicina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
cytochrome c
-
Thiocapsa roseopersicina
0.05
-
AMP reaction with cytochrome c Thiocapsa roseopersicina
0.073
-
sulfite reaction with ferricyanide Thiocapsa roseopersicina
0.093
-
sulfite reaction with cytochrome c Thiocapsa roseopersicina
0.13
-
ferricyanide
-
Thiocapsa roseopersicina
1.5
-
sulfite reaction with ferricyanide Thiocapsa roseopersicina

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains 4 mol of non-heme iron per mol of enzyme Thiocapsa roseopersicina

Organism

Organism UniProt Comment Textmining
Thiocapsa roseopersicina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thiocapsa roseopersicina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Thiocapsa roseopersicina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + sulfite + cytochrome c
-
Thiocapsa roseopersicina adenylyl sulfate + ?
-
?
AMP + sulfite + ferricyanide
-
Thiocapsa roseopersicina adenylyl sulfate + ferrocyanide
-
?
CMP + sulfite + cytochrome c 7% of the activity with AMP Thiocapsa roseopersicina cytidylyl sulfate + ?
-
?
CMP + sulfite + ferricyanide 8% of the activity with AMP Thiocapsa roseopersicina cytidylyl sulfate + ferrocyanide
-
?
dAMP + sulfite + cytochrome c 80% of the activity with AMP Thiocapsa roseopersicina deoxyadenylyl sulfate + ?
-
?
deoxyAMP + sulfite + ferricyanide 128% of maximal activity Thiocapsa roseopersicina deoxyadenylyl sulfate + ferrocyanide
-
?
GMP + sulfite + cytochrome c 9% of the activity with AMP Thiocapsa roseopersicina guanylyl sulfate + ?
-
?
GMP + sulfite + ferricyanide 51% of the activity with AMP Thiocapsa roseopersicina guanylyl sulfate + ferrocyanide
-
?
IMP + sulfite + cytochrome c 62% of the activity with AMP Thiocapsa roseopersicina ?
-
?
IMP + sulfite + ferricyanide 90% of the activity with AMP Thiocapsa roseopersicina ? + ferrocyanide
-
?
UMP + sulfite + cytochrome c 9% of the activity with AMP Thiocapsa roseopersicina uridylyl sulfate + ?
-
?
UMP + sulfite + ferricyanide 8% of the activity with AMP Thiocapsa roseopersicina uridylyl sulfate + ferrocyanide
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
reaction with ferricyanide Thiocapsa roseopersicina
9
-
reaction with cytochrome c Thiocapsa roseopersicina

Cofactor

Cofactor Comment Organism Structure
FAD enzyme contains 1 mol of FAD per mol of enzyme Thiocapsa roseopersicina
heme contains 2 heme groups of cytochrome c characterper mol of enzyme Thiocapsa roseopersicina