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Literature summary for 1.8.99.2 extracted from

  • Stille, W.; Trueper, H.G.
    Adenylylsulfate reductase in some new sulfate-reducing bacteria (1984), Arch. Microbiol., 137, 145-150.
No PubMed abstract available
Search for more literature for 1.8.99.2

Inhibitors

Inhibitors Comment Organism Structure
AMP inhibits reaction with cytochrome c above 0.1 mM, inhibits reaction with ferricyanide above 1.6 mM Desulfobulbus propionicus
AMP
-
 Desulfovibrio vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 cytochrome c
-
 Desulfobulbus propionicus
0.071
-
 sulfite reaction with cytochrome c Desulfobulbus propionicus
0.089
-
 ferricyanide
-
 Desulfobulbus propionicus
0.09
-
 AMP reaction with ferricyanide Desulfobulbus propionicus
0.091
-
 AMP reaction with cytochrome c Desulfobulbus propionicus
1.3
-
 sulfite reaction with ferricyanide Desulfobulbus propionicus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron
-
 Desulfovibrio vulgaris
Iron contains 8 mol of iron per mol of enzyme Desulfobulbus propionicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
175000
-
 gel filtration Desulfobulbus propionicus

Organism

Organism UniProt Comment Textmining
Desulfobacter postgatei
-
-
-
Desulfobulbus propionicus
-
-
-
Desulfococcus multivorans
-
-
-
Desulfosarcina variabilis
-
-
-
Desulfovibrio vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Desulfobulbus propionicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.93
-
-
 Desulfobulbus propionicus

Storage Stability

Storage Stability Organism
-18°C, 88-90% loss of activity after 1 week, 68% loss of activity after 16 days, in presence of 50% glycerol, 46% loss of activity after 16 days Desulfobulbus propionicus
4°C, more than 20% loss of activity after 8 h Desulfobulbus propionicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + sulfite + ferricyanide
-
 Desulfobulbus propionicus ? + ferrocyanide
-
 ?
AMP + sulfite + cytochrome c
-
 Desulfovibrio vulgaris adenylyl sulfate + ?
-
 ?
AMP + sulfite + cytochrome c reaction with horse heart cytochrome c, no activity with cytochrome c from Candida krusei Desulfobulbus propionicus adenylyl sulfate + ?
-
 ?
AMP + sulfite + ferricyanide
-
 Desulfovibrio vulgaris adenylyl sulfate + ferrocyanide
-
 ?
AMP + sulfite + ferricyanide
-
 Desulfobulbus propionicus adenylyl sulfate + ferrocyanide
-
 ?
ATP + sulfite + ferricyanide 12.8% of the activity with AMP Desulfobulbus propionicus ? + ferrocyanide
-
 ?
CMP + sulfite + ferricyanide
-
 Desulfovibrio vulgaris cytidylyl sulfate + ferrocyanide
-
 ?
CMP + sulfite + ferricyanide 7.6% of the activity with AMP Desulfobulbus propionicus cytidylyl sulfate + ferrocyanide
-
 ?
deoxyAMP + sulfite + ferricyanide 82.7% of the activity with AMP Desulfobulbus propionicus deoxyadenylyl sulfate + ferrocyanide
-
 ?
GMP + sulfite + ferricyanide
-
 Desulfovibrio vulgaris guanylyl sulfate + ferrocyanide
-
 ?
GMP + sulfite + ferricyanide 29.4% of the activity with AMP Desulfobulbus propionicus guanylyl sulfate + ferrocyanide
-
 ?
UMP + sulfite + ferricyanide
-
 Desulfovibrio vulgaris uridylyl sulfate + ferrocyanide
-
 ?
UMP + sulfite + ferricyanide 7.6% of the activity with AMP Desulfobulbus propionicus uridylyl sulfate + ferrocyanide
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 5 min, complete loss of activity Desulfobulbus propionicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 reaction with ferricyanide Desulfobulbus propionicus
8.8
-
 reaction with cytochrome c Desulfobulbus propionicus

Cofactor

Cofactor Comment Organism Structure
flavin contains 1 mol of flavin per mol of enzyme Desulfobulbus propionicus