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Literature summary for 1.8.98.3 extracted from

  • Johnson, E.F.; Mukhopadhyay, B.
    A new type of sulfite reductase, a novel coenzyme F420-dependent enzyme, from the methanarchaeon Methanocaldococcus jannaschii (2005), J. Biol. Chem., 280, 38776-38786.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
sulfite pH 7.0, temperature not specified in the publication Methanocaldococcus jannaschii
0.021
-
reduced coenzyme F420 pH 7.0, temperature not specified in the publication Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, SDS-PAGE Methanocaldococcus jannaschii
350000
-
gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sulfite + reduced coenzyme F420 Methanocaldococcus jannaschii sulfite detoxification and assimilation mechanisms hydrogen sulfide + oxidized coenzyme F420
-
?
sulfite + reduced coenzyme F420 Methanocaldococcus jannaschii DSM 2661 sulfite detoxification and assimilation mechanisms hydrogen sulfide + oxidized coenzyme F420
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58280
-
-
Methanocaldococcus jannaschii DSM 2661 Q58280
-
-

Oxidation Stability

Oxidation Stability Organism
activity in cell extracts is highly oxygen sensitive. About 92% activity is lost if the cell extract is exposed to air for 60 min. Dithiothreitol and 2-mercapotoethanol neither protect the enzyme from inactivation nor restore activity in an air-inactivated enzyme preparation Methanocaldococcus jannaschii

Purification (Commentary)

Purification (Comment) Organism
-
Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.09
-
pH 7.0, temperature not specified in the publication, substrate: sulfite and reduced methyl viologen Methanocaldococcus jannaschii
1.3
-
pH 7.0, temperature not specified in the publication, cell extract, substrate: sulfite and reduced coenzyme F420 Methanocaldococcus jannaschii
18.2
-
pH 7.0, temperature not specified in the publication, purified enzyme, substrate: sulfite and reduced coenzyme F420 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + reduced coenzyme F420 sulfite detoxification and assimilation mechanisms Methanocaldococcus jannaschii hydrogen sulfide + oxidized coenzyme F420
-
?
sulfite + reduced coenzyme F420 no activity with: thiosulfate, sulfate, NADH and NADPH Methanocaldococcus jannaschii hydrogen sulfide + oxidized coenzyme F420
-
?
sulfite + reduced coenzyme F420 sulfite detoxification and assimilation mechanisms Methanocaldococcus jannaschii DSM 2661 hydrogen sulfide + oxidized coenzyme F420
-
?
sulfite + reduced coenzyme F420 no activity with: thiosulfate, sulfate, NADH and NADPH Methanocaldococcus jannaschii DSM 2661 hydrogen sulfide + oxidized coenzyme F420
-
?
sulfite + reduced methyl viologen no activity with: thiosulfate, sulfate, NADH and NADPH Methanocaldococcus jannaschii hydrogen sulfide + oxidized methyl viologen
-
?
sulfite + reduced methyl viologen no activity with: thiosulfate, sulfate, NADH and NADPH Methanocaldococcus jannaschii DSM 2661 hydrogen sulfide + oxidized methyl viologen
-
?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
coenzyme F420-dependent sulfite reductase
-
Methanocaldococcus jannaschii
Fsr
-
Methanocaldococcus jannaschii
MJ0870 locus name Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Methanocaldococcus jannaschii

Cofactor

Cofactor Comment Organism Structure
siroheme the N-terminal half of the 70-kDa Fsr polypeptide represents a H2F420 dehydrogenase and the C-terminal half a dissimilatory-type siroheme sulfite reductase Methanocaldococcus jannaschii

Expression

Organism Comment Expression
Methanocaldococcus jannaschii sulfite induces coenzyme F420-dependent sulfite reductase up

General Information

General Information Comment Organism
physiological function sulfite detoxification and assimilation mechanisms Methanocaldococcus jannaschii