KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
sulfite | pH 7.0, temperature not specified in the publication | Methanocaldococcus jannaschii | |
0.021 | - |
reduced coenzyme F420 | pH 7.0, temperature not specified in the publication | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, SDS-PAGE | Methanocaldococcus jannaschii |
350000 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + reduced coenzyme F420 | Methanocaldococcus jannaschii | sulfite detoxification and assimilation mechanisms | hydrogen sulfide + oxidized coenzyme F420 | - |
? | |
sulfite + reduced coenzyme F420 | Methanocaldococcus jannaschii DSM 2661 | sulfite detoxification and assimilation mechanisms | hydrogen sulfide + oxidized coenzyme F420 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58280 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58280 | - |
- |
Oxidation Stability | Organism |
---|---|
activity in cell extracts is highly oxygen sensitive. About 92% activity is lost if the cell extract is exposed to air for 60 min. Dithiothreitol and 2-mercapotoethanol neither protect the enzyme from inactivation nor restore activity in an air-inactivated enzyme preparation | Methanocaldococcus jannaschii |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.09 | - |
pH 7.0, temperature not specified in the publication, substrate: sulfite and reduced methyl viologen | Methanocaldococcus jannaschii |
1.3 | - |
pH 7.0, temperature not specified in the publication, cell extract, substrate: sulfite and reduced coenzyme F420 | Methanocaldococcus jannaschii |
18.2 | - |
pH 7.0, temperature not specified in the publication, purified enzyme, substrate: sulfite and reduced coenzyme F420 | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + reduced coenzyme F420 | sulfite detoxification and assimilation mechanisms | Methanocaldococcus jannaschii | hydrogen sulfide + oxidized coenzyme F420 | - |
? | |
sulfite + reduced coenzyme F420 | no activity with: thiosulfate, sulfate, NADH and NADPH | Methanocaldococcus jannaschii | hydrogen sulfide + oxidized coenzyme F420 | - |
? | |
sulfite + reduced coenzyme F420 | sulfite detoxification and assimilation mechanisms | Methanocaldococcus jannaschii DSM 2661 | hydrogen sulfide + oxidized coenzyme F420 | - |
? | |
sulfite + reduced coenzyme F420 | no activity with: thiosulfate, sulfate, NADH and NADPH | Methanocaldococcus jannaschii DSM 2661 | hydrogen sulfide + oxidized coenzyme F420 | - |
? | |
sulfite + reduced methyl viologen | no activity with: thiosulfate, sulfate, NADH and NADPH | Methanocaldococcus jannaschii | hydrogen sulfide + oxidized methyl viologen | - |
? | |
sulfite + reduced methyl viologen | no activity with: thiosulfate, sulfate, NADH and NADPH | Methanocaldococcus jannaschii DSM 2661 | hydrogen sulfide + oxidized methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
coenzyme F420-dependent sulfite reductase | - |
Methanocaldococcus jannaschii |
Fsr | - |
Methanocaldococcus jannaschii |
MJ0870 | locus name | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
siroheme | the N-terminal half of the 70-kDa Fsr polypeptide represents a H2F420 dehydrogenase and the C-terminal half a dissimilatory-type siroheme sulfite reductase | Methanocaldococcus jannaschii |
Organism | Comment | Expression |
---|---|---|
Methanocaldococcus jannaschii | sulfite induces coenzyme F420-dependent sulfite reductase | up |
General Information | Comment | Organism |
---|---|---|
physiological function | sulfite detoxification and assimilation mechanisms | Methanocaldococcus jannaschii |