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Literature summary for 1.8.98.1 extracted from
- Methanobacterium thermoautotrophicum contains a soluble enzyme system that specifically catalyzes the reduction of the heterodisulfide of coenzyme M and 7-mercaptoheptanoylthreonine phosphate with H2 (1988), FEBS Lett., 234, 223-227.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-value for N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine is below 0.1 mM | Methanothermobacter thermautotrophicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Methanothermobacter thermautotrophicus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermobacter thermautotrophicus | - |
- |
- |
| Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermobacter thermautotrophicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.2 | - |
- |
Methanothermobacter thermautotrophicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, under N2, partially purified enzyme, 50 mM Tris-HCl, pH 7.6, 0.5 M NaCl, loss of 20% activity within 24 h | Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | - |
Methanothermobacter thermautotrophicus | coenzyme B + coenzyme M | - |
? |  |
| N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | coenzyme B + coenzyme M | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
rapid inactivation above | Methanothermobacter thermautotrophicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
Pipes/KOH buffer | Methanothermobacter thermautotrophicus |
| 7.6 | - |
20°C, Tris-HCl buffer | Methanothermobacter thermautotrophicus |
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