Protein Variants | Comment | Organism |
---|---|---|
C27S | mutation in truncated, stabilized FTR mutant lacking 24 N-terminal amino acids. Mutant C27S is perfectly capable of activating FBPase and shows a thioredoxin f-concentration dependency comparable to the FTR truncation mutant | Spinacia oleracea |
C84S | mutation in truncated, stabilized FTR mutant lacking 24 N-terminal amino acids. Mutant C84S is produced at about the same level as the WT protein, it is extremely labile and disintegrates very rapidly during the first steps of purifiation | Spinacia oleracea |
additional information | construction two N-terminal truncation mutants by removing 16 or 24 residues from the variable subunit. The mutant proteins are readily expressed and show half-saturation values for ferredoxin and thioredoxin f comparable to wild-type. Truncation increases significantly their stability | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | P38365 | variable subunit | - |