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Literature summary for 1.8.7.1 extracted from
- Characterization of two ferredoxin-dependent sulfite reductases having different substrate specificity in the red alga Cyanidioschyzon merolae (2017), J. Biochem., 162, 37-43 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Cyanidioschyzon merolae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G212S/L213T/Y214L/S217C/C220I/S221N | mutations mimic partially isoform SiRA | Cyanidioschyzon merolae |
| S217C | mutation mimics the corresponding residue in isoform SiRA, recovers sulfite reduction activity | Cyanidioschyzon merolae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0064 | - |
sulfite | mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication | Cyanidioschyzon merolae |  |
| 0.009 | - |
sulfite | truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication | Cyanidioschyzon merolae | |
| 0.028 | - |
sulfite | truncated protein, residues 19-731, pH not specified in the publication, temperature not specified in the publication | Cyanidioschyzon merolae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyanidioschyzon merolae | M1URK1 | isoform SiRA | - |
| Cyanidioschyzon merolae | M1V771 | isoform SiRB | - |
| Cyanidioschyzon merolae 10D | M1URK1 | isoform SiRA | - |
| Cyanidioschyzon merolae 10D | M1V771 | isoform SiRB | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme additionally shows activity of nitrite reductase, EC 1.7.7.1 | Cyanidioschyzon merolae | ? | - |
? | |
| additional information | enzyme additionally shows activity of nitrite reductase, EC 1.7.7.1 | Cyanidioschyzon merolae 10D | ? | - |
? | |
| sulfite + reduced ferredoxin + H+ | - |
Cyanidioschyzon merolae | hydrogen sulfide + oxidized ferredoxin + H2O | - |
? | |
| sulfite + reduced ferredoxin + H+ | - |
Cyanidioschyzon merolae 10D | hydrogen sulfide + oxidized ferredoxin + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYME_CMG021C | - |
Cyanidioschyzon merolae |
| CYME_CMJ117C | - |
Cyanidioschyzon merolae |
| SiRA | - |
Cyanidioschyzon merolae |
| SirB | - |
Cyanidioschyzon merolae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.078 | - |
sulfite | truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication | Cyanidioschyzon merolae | |
| 0.133 | - |
sulfite | mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication | Cyanidioschyzon merolae | |
| 1.93 | - |
sulfite | truncated protein, residues 19-731, pH not specified in the publication, temperature not specified in the publication | Cyanidioschyzon merolae | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Cyanidioschyzon merolae | enzyme is constitutively expressed | additional information |
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