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Literature summary for 1.8.7.1 extracted from

  • Saitoh, T.; Ikegami, T.; Nakayama, M.; Teshima, K.; Akutsu, H.; Hase, T.
    NMR study of the electron transfer complex of plant ferredoxin and sulfite reductase: mapping the interaction sites of ferredoxin (2006), J. Biol. Chem., 281, 10482-10488.
    View publication on PubMed
Search for more literature for 1.8.7.1

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 reduced ferredoxin using wild type ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays
0.0062
-
 reduced ferredoxin using E92Q/E93Q mutant ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays
0.0095
-
 reduced ferredoxin using E92Q/E30Q/D34N mutant ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays
0.0147
-
 reduced ferredoxin using E92Q/E93Q/E29Q/D34N mutant ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
4Fe-4S
-
 Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 1 * 65000 Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + reduced ferredoxin
-
 Zea mays hydrogen sulfide + oxidized ferredoxin + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65000 Zea mays

Synonyms

Synonyms Comment Organism
ferredoxin and sulfite reductase
-
 Zea mays
SIR
-
 Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 reduced ferredoxin using E92Q/E93Q/E29Q/D34N mutant ferredoxin, the reaction mixture contains regulator of fumarate and nitrate reduction (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays
2.66
-
 reduced ferredoxin using E92Q/E30Q/D34N mutant ferredoxin, the reaction mixture contains regulator of fumarate and nitrate reduction (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays
2.7
-
 reduced ferredoxin using E92Q/E93Q mutant ferredoxin, the reaction mixture contains regulator of fumarate and nitrate reduction (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30-mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays
3.65
-
 reduced ferredoxin using wild type ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl Zea mays

Cofactor

Cofactor Comment Organism Structure
siroheme
-
 Zea mays