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Literature summary for 1.8.5.9 extracted from

  • Yazawa, K.; Furusawa, H.; Okahata, Y.
    Real-time monitoring of intermediates reveals the reaction pathway in the thiol-disulfide exchange between disulfide bond formation protein A (DsbA) and B (DsbB) on a membrane-immobilized quartz crystal microbalance (QCM) system (2013), J. Biol. Chem., 288, 35969-35981 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.5.9

Cloned(Commentary)

Cloned (Comment) Organism
biotin-labelled enzyme is expressed in Escherichia coli C41 (DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
C41A/C44A the mutant has two cysteine residues at Cys104 and Cys130 compared to the wild type enzyme with Cys41, Cys44, Cys104 and Cys130 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
inner membrane
-
 Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[DsbA protein] with reduced L-cysteine residues + ubiquinone Escherichia coli overall reaction [DsbA protein] carrying a disulfide bond + ubiquinol
-
 ?
[DsbA protein] with reduced L-cysteine residues + [DsbB protein] carrying a disulfide bond Escherichia coli
-
 [DsbA protein] carrying a disulfide bond + [DsbB protein] with reduced L-cysteine residues
-
 ?
[DsbB protein] with reduced L-cysteine residues + ubiquinone Escherichia coli
-
 [DsbB protein] carrying a disulfide bond + ubiquinol
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[DsbA protein] with reduced L-cysteine residues + ubiquinone overall reaction Escherichia coli [DsbA protein] carrying a disulfide bond + ubiquinol
-
 ?
[DsbA protein] with reduced L-cysteine residues + [DsbB protein] carrying a disulfide bond
-
 Escherichia coli [DsbA protein] carrying a disulfide bond + [DsbB protein] with reduced L-cysteine residues
-
 ?
[DsbB protein] with reduced L-cysteine residues + ubiquinone
-
 Escherichia coli [DsbB protein] carrying a disulfide bond + ubiquinol
-
 ?

Synonyms

Synonyms Comment Organism
disulfide bond formation protein B
-
 Escherichia coli
DsbB
-
 Escherichia coli