Cloned (Comment) | Organism |
---|---|
expressed with the biotin carboxyl carrier protein tag in Escherichia coli C41 (DE3) cells | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [DsbA protein] with reduced L-cysteine residues + ubiquinone | Escherichia coli | overall reaction | a [DsbA protein] carrying a disulfide bond + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 pg gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [DsbA protein] with reduced L-cysteine residues + ubiquinone | overall reaction | Escherichia coli | a [DsbA protein] carrying a disulfide bond + ubiquinol | - |
? | |
additional information | enzyme-catalyzed DsbA protein oxidation is an entropy-driven reaction originating from delocalization of electrons among four cysteine residues in the enzyme, which proceeds independently of an oxidizing power of ubiquinone | Escherichia coli | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 20000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
disulfide-bond formation protein B | - |
Escherichia coli |
DsbB | - |
Escherichia coli |