Literature summary for 1.8.5.7 extracted from

Di Matteo, A.; Federici, L.; Masulli, M.; Carletti, E.; Santorelli, D.; Cassidy, J.; Paradisi, F.; Di Ilio, C.; Allocati, N.
Structural characterization of the Xi class glutathione transferase from the haloalkaliphilic archaeon Natrialba magadii (2019), Front. Microbiol., 10, 9 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene nmagghr, a single gene, synthesis of a cDNA from the total RNA extracted from Natrialba magadii strain ATCC 43099, sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli	Natrialba magadii

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme in apoform, mixing of 0.5 ml of 6.6 mg/ml protein in 50 mM Tris-HCl, pH 7.5, and 3.5 M NaCl, with 0.5 ml of reservoir solution containing 25% w/v PEG 3350, 0.1 M Tris-HCl, pH 8.5, and 0.2 M lithium sulfate, at 21°C, X-ray diffraction structure determination and analysis at 2.61 A resolution, molecular replacement using the structure of monomeric putative GST from Corynebacterium glutamicum (PDB ID 3M1G) as a search model, model building	Natrialba magadii

Crystallization (Comment)

Organism

purified enzyme in apoform, mixing of 0.5 ml of 6.6 mg/ml protein in 50 mM Tris-HCl, pH 7.5, and 3.5 M NaCl, with 0.5 ml of reservoir solution containing 25% w/v PEG 3350, 0.1 M Tris-HCl, pH 8.5, and 0.2 M lithium sulfate, at 21°C, X-ray diffraction structure determination and analysis at 2.61 A resolution, molecular replacement using the structure of monomeric putative GST from Corynebacterium glutamicum (PDB ID 3M1G) as a search model, model building

Natrialba magadii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
77000	-	recombinant N-terminally His6-tagged enzyme, gel filtration	Natrialba magadii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii	-	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii ATCC 43099	-	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii NBRC 102185	-	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii DSM 3394	-	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii CCM 3739	-	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii NCIMB 2190	-	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii JCM 8861	-	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii CIP 104546	-	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii MS3	-	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii IAM 13178	-	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii	-	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii ATCC 43099	-	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii NBRC 102185	-	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii DSM 3394	-	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii CCM 3739	-	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii NCIMB 2190	-	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii JCM 8861	-	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii CIP 104546	-	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii MS3	-	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	Natrialba magadii IAM 13178	-	glutathione disulfide + hydroquinone	-	?

Organism

Organism	UniProt	Comment	Textmining
Natrialba magadii	D3SS28	-	-
Natrialba magadii ATCC 43099	D3SS28	-	-
Natrialba magadii CCM 3739	D3SS28	-	-
Natrialba magadii CIP 104546	D3SS28	-	-
Natrialba magadii DSM 3394	D3SS28	-	-
Natrialba magadii IAM 13178	D3SS28	-	-
Natrialba magadii JCM 8861	D3SS28	-	-
Natrialba magadii MS3	D3SS28	-	-
Natrialba magadii NBRC 102185	D3SS28	-	-
Natrialba magadii NCIMB 2190	D3SS28	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli	Natrialba magadii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii	?	-	?
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii ATCC 43099	?	-	?
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii NBRC 102185	?	-	?
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii DSM 3394	?	-	?
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii CCM 3739	?	-	?
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii NCIMB 2190	?	-	?
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii JCM 8861	?	-	?
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii CIP 104546	?	-	?
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii MS3	?	-	?
1,4-benzoquinone + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii IAM 13178	?	-	?
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene	-	Natrialba magadii	?	-	?
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene	-	Natrialba magadii ATCC 43099	?	-	?
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene	-	Natrialba magadii NBRC 102185	?	-	?
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene	-	Natrialba magadii DSM 3394	?	-	?
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene	-	Natrialba magadii CCM 3739	?	-	?
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene	-	Natrialba magadii NCIMB 2190	?	-	?
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene	-	Natrialba magadii JCM 8861	?	-	?
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene	-	Natrialba magadii CIP 104546	?	-	?
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene	-	Natrialba magadii MS3	?	-	?
gamma-glutamylcysteine + 1-chloro-2,4-dinitrobenzene	-	Natrialba magadii IAM 13178	?	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii ATCC 43099	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii NBRC 102185	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii DSM 3394	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii CCM 3739	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii NCIMB 2190	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii JCM 8861	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii CIP 104546	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii MS3	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii IAM 13178	gamma-glutamylcysteine glutahione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii	gamma-glutamylcysteine glutathione disulfide + hydroquinone	-	?
gamma-glutamylcysteine + ethacrynic acid	-	Natrialba magadii	?	-	?
glutathione + 1,4-benzoquinone	-	Natrialba magadii	?	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii ATCC 43099	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii NBRC 102185	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii DSM 3394	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii CCM 3739	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii NCIMB 2190	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii JCM 8861	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii CIP 104546	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii MS3	glutathione disulfide + hydroquinone	-	?
glutathione + 2-(glutathione-S-yl)-hydroquinone	-	Natrialba magadii IAM 13178	glutathione disulfide + hydroquinone	-	?
glutathione + 2-hydroxyethyldisulfide	-	Natrialba magadii	?	-	?
glutathione + dehydroascorbic acid	-	Natrialba magadii	?	-	?
additional information	the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate	Natrialba magadii	?	-	-
additional information	the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate	Natrialba magadii ATCC 43099	?	-	-
additional information	the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate	Natrialba magadii NBRC 102185	?	-	-
additional information	the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate	Natrialba magadii DSM 3394	?	-	-
additional information	the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate	Natrialba magadii CCM 3739	?	-	-
additional information	the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate	Natrialba magadii NCIMB 2190	?	-	-
additional information	the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate	Natrialba magadii JCM 8861	?	-	-
additional information	the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate	Natrialba magadii CIP 104546	?	-	-
additional information	the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate	Natrialba magadii MS3	?	-	-
additional information	the enzyme shows preference for gamma-glutamyl-cysteine over glutathione as a reducing thiol. The main activity of Xi class GSTs is thought to be the S-glutathionyl-hydroquinone thiol-dependent reduction, which is monitored using benzoquinone as a model substrate	Natrialba magadii IAM 13178	?	-	-

Subunits

Subunits	Comment	Organism
?	x * 38000, recombinant N-terminally His6-tagged enzyme, SDS-PAGE	Natrialba magadii
More	structure analysis of enzyme NmGHR, detailed overview	Natrialba magadii

Synonyms

Synonyms	Comment	Organism
nmagghr	-	Natrialba magadii
Nmag_1170	-	Natrialba magadii
NmGHR	-	Natrialba magadii
Xi class glutathione transferase	-	Natrialba magadii
Xi class GST	-	Natrialba magadii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	40	assay at	Natrialba magadii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	8	assay at	Natrialba magadii

General Information

General Information	Comment	Organism
evolution	Xi class glutathione transferases (GSTs) are a group, within this large superfamily of enzymes, specifically endowed with glutathione-dependent reductase activity on glutathionyl-hydroquinone. Enzymes belonging to this group are widely distributed in bacteria, fungi, and plants but not in higher eukaryotes. Xi class GSTs are also frequently found in archaea. Multiple alignment analysis of NmGHR in comparison with other Xi class GSTs show that all sequences display several common motifs. All members exhibit a strictly conserved CPWA motif that could play a key role in the binding of GS-(hydro)-quinones. Phylogenetic analysis, members of prokaryotic and eukaryotic Xi class GSTs and their homologous belonging to different GST classes are characterized by the presence of a cysteine residue in the G-site, overview	Natrialba magadii
additional information	enzyme NmGHR displays is enriched in negatively charged residues, which are evenly distributed along the surface of the protein, and is characterized by a peculiar distribution of hydrophobic residues. The thiol binding site (G-site) of the enzyme is well suited for hosting gamma-glutamyl-cysteine. Circular dichroism analysis of NmGHR	Natrialba magadii