BRENDA - Enzyme Database
show all sequences of 1.8.5.7

Crystal structure of Saccharomyces cerevisiae ECM4, a Xi-class glutathione transferase that reacts with glutathionyl-(hydro)quinones

Schwartz, M.; Didierjean, C.; Hecker, A.; Girardet, J.M.; Morel-Rouhier, M.; Gelhaye, E.; Favier, F.; PLoS ONE 11, e0164678 (2016)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
isoform ECM4 shows extensions including a huge loop which contributes to the quaternary assembly. Soaking of ECM4 crystals with glutathionyl-menadione results in a structure where glutathione forms a mixed disulfide bond with the cysteine 46. Residues H345 and H350, F228, Y224 and W48 could play crucial roles in binding of glutathionyl-(hydro)quinones, and in assisting C46 during catalysis
Saccharomyces cerevisiae
Organism
Organism
UniProt
Commentary
Textmining
Saccharomyces cerevisiae
P36156
cf. EC 1.8.5.1, EC 2.5.1.18
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
glutathione + 2-(glutathione-S-yl)-menadione
-
741262
Saccharomyces cerevisiae
glutathione disulfide + menadione
-
-
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?
Synonyms
Synonyms
Commentary
Organism
ECM4
-
Saccharomyces cerevisiae
Crystallization (Commentary) (protein specific)
Crystallization
Organism
isoform ECM4 shows extensions including a huge loop which contributes to the quaternary assembly. Soaking of ECM4 crystals with glutathionyl-menadione results in a structure where glutathione forms a mixed disulfide bond with the cysteine 46. Residues H345 and H350, F228, Y224 and W48 could play crucial roles in binding of glutathionyl-(hydro)quinones, and in assisting C46 during catalysis
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
glutathione + 2-(glutathione-S-yl)-menadione
-
741262
Saccharomyces cerevisiae
glutathione disulfide + menadione
-
-
-
?
Other publictions for EC 1.8.5.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741262
Schwartz
Crystal structure of Saccharom ...
Saccharomyces cerevisiae
PLoS ONE
11
e0164678
2016
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740452
Lallement
Glutathionyl-hydroquinone redu ...
Populus trichocarpa
FEBS Lett.
589
37-44
2015
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2
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1
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5
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740055
Lam
Reduction of benzoquinones to ...
Cupriavidus necator, Saccharomyces cerevisiae, Sphingobium chlorophenolicum, Escherichia coli, Sphingobium chlorophenolicum ATCC 29327, Cupriavidus necator JMP 134-1
Biochemistry
51
5014-5021
2012
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20
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740704
Green
Structural understanding of th ...
Escherichia coli
J. Biol. Chem.
287
35838-35848
2012
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4
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740012
Xun
S-Glutathionyl-(chloro)hydroqu ...
Sphingobium chlorophenolicum
Biochem. J.
428
419-427
2010
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740614
Huang
Maintenance role of a glutathi ...
Sphingobium chlorophenolicum, Sphingobium chlorophenolicum ATCC 39723
J. Bacteriol.
190
7595-7600
2008
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