BRENDA - Enzyme Database
show all sequences of 1.8.5.7

Glutathionyl-hydroquinone reductases from poplar are plastidial proteins that deglutathionylate both reduced and oxidized glutathionylated quinones

Lallement, P.; Meux, E.; Gualberto, J.; Dumarcay, S.; Favier, F.; Didierjean, C.; Saul, F.; Haouz, A.; Morel-Rouhier, M.; Gelhaye, E.; Rouhier, N.; Hecker, A.; FEBS Lett. 589, 37-44 (2015)
No PubMed abstract available

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
structure in complex with glutathione, to 2.5 A resolution. Isoform GHR1 adopts a typical glutathionyl-hydroquinone reductase fold, with a dimerization interface comparable to that of the bacterial and fungal counterparts
Populus trichocarpa
Engineering
Protein Variants
Commentary
Organism
C49A
mutation of catalytic cysteine, loss of activity
Populus trichocarpa
C49S
mutation of catalytic cysteine, loss of activity
Populus trichocarpa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0027
-
S-glutathionylquercetin
wild-type, pH 8.0, 20°C
Populus trichocarpa
0.003
-
2-(glutathione-S-yl)-menadiol
wild-type, pH 8.0, 20°C
Populus trichocarpa
0.072
-
(glutathione-S-yl)-2-chloro-1,4-benzoquinone
wild-type, pH 8.0, 20°C
Populus trichocarpa
0.0926
-
2-(glutathione-S-yl)-1,4-benzoquinone
wild-type, pH 8.0, 20°C
Populus trichocarpa
0.27
-
2-(glutathione-S-yl)-menadione
wild-type, pH 8.0, 20°C
Populus trichocarpa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
-
Populus trichocarpa
9536
-
Organism
Organism
UniProt
Commentary
Textmining
Populus trichocarpa
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
primarily expressed in reproductive organs
Populus trichocarpa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
glutathione + (glutathione-S-yl)-2-chloro-1,4-benzoquinone
-
740452
Populus trichocarpa
glutathione disulfide + 2-chloro-1,4-benzoquinone
-
-
-
?
glutathione + 2'-(glutathione-S-yl)-quercetin
-
740452
Populus trichocarpa
glutathione disulfide + quercetin
-
-
-
?
glutathione + 2-(glutathione-S-yl)-1,4-benzoquinone
-
740452
Populus trichocarpa
glutathione disulfide + 1,4-benzoquinone
-
-
-
?
glutathione + 2-(glutathione-S-yl)-menadiol
-
740452
Populus trichocarpa
glutathione disulfide + menadiol
-
-
-
?
glutathione + 2-(glutathione-S-yl)-menadione
-
740452
Populus trichocarpa
glutathione disulfide + menadione
-
-
-
?
additional information
isoform GHR1 catalyzes the deglutathionylation of both reduced and oxidized glutathionylated quinones, but the enzyme is more catalytically efficient with the reduced forms
740452
Populus trichocarpa
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
GHR1
-
Populus trichocarpa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.013
-
S-glutathionylquercetin
wild-type, pH 8.0, 20°C
Populus trichocarpa
1.62
-
2-(glutathione-S-yl)-1,4-benzoquinone
wild-type, pH 8.0, 20°C
Populus trichocarpa
4.75
-
2-(glutathione-S-yl)-menadione
wild-type, pH 8.0, 20°C
Populus trichocarpa
7.96
-
(glutathione-S-yl)-2-chloro-1,4-benzoquinone
wild-type, pH 8.0, 20°C
Populus trichocarpa
9.42
-
2-(glutathione-S-yl)-menadiol
wild-type, pH 8.0, 20°C
Populus trichocarpa
Crystallization (Commentary) (protein specific)
Crystallization
Organism
structure in complex with glutathione, to 2.5 A resolution. Isoform GHR1 adopts a typical glutathionyl-hydroquinone reductase fold, with a dimerization interface comparable to that of the bacterial and fungal counterparts
Populus trichocarpa
Engineering (protein specific)
Protein Variants
Commentary
Organism
C49A
mutation of catalytic cysteine, loss of activity
Populus trichocarpa
C49S
mutation of catalytic cysteine, loss of activity
Populus trichocarpa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0027
-
S-glutathionylquercetin
wild-type, pH 8.0, 20°C
Populus trichocarpa
0.003
-
2-(glutathione-S-yl)-menadiol
wild-type, pH 8.0, 20°C
Populus trichocarpa
0.072
-
(glutathione-S-yl)-2-chloro-1,4-benzoquinone
wild-type, pH 8.0, 20°C
Populus trichocarpa
0.0926
-
2-(glutathione-S-yl)-1,4-benzoquinone
wild-type, pH 8.0, 20°C
Populus trichocarpa
0.27
-
2-(glutathione-S-yl)-menadione
wild-type, pH 8.0, 20°C
Populus trichocarpa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
-
Populus trichocarpa
9536
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
primarily expressed in reproductive organs
Populus trichocarpa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
glutathione + (glutathione-S-yl)-2-chloro-1,4-benzoquinone
-
740452
Populus trichocarpa
glutathione disulfide + 2-chloro-1,4-benzoquinone
-
-
-
?
glutathione + 2'-(glutathione-S-yl)-quercetin
-
740452
Populus trichocarpa
glutathione disulfide + quercetin
-
-
-
?
glutathione + 2-(glutathione-S-yl)-1,4-benzoquinone
-
740452
Populus trichocarpa
glutathione disulfide + 1,4-benzoquinone
-
-
-
?
glutathione + 2-(glutathione-S-yl)-menadiol
-
740452
Populus trichocarpa
glutathione disulfide + menadiol
-
-
-
?
glutathione + 2-(glutathione-S-yl)-menadione
-
740452
Populus trichocarpa
glutathione disulfide + menadione
-
-
-
?
additional information
isoform GHR1 catalyzes the deglutathionylation of both reduced and oxidized glutathionylated quinones, but the enzyme is more catalytically efficient with the reduced forms
740452
Populus trichocarpa
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.013
-
S-glutathionylquercetin
wild-type, pH 8.0, 20°C
Populus trichocarpa
1.62
-
2-(glutathione-S-yl)-1,4-benzoquinone
wild-type, pH 8.0, 20°C
Populus trichocarpa
4.75
-
2-(glutathione-S-yl)-menadione
wild-type, pH 8.0, 20°C
Populus trichocarpa
7.96
-
(glutathione-S-yl)-2-chloro-1,4-benzoquinone
wild-type, pH 8.0, 20°C
Populus trichocarpa
9.42
-
2-(glutathione-S-yl)-menadiol
wild-type, pH 8.0, 20°C
Populus trichocarpa
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.95
-
S-glutathionylquercetin
wild-type, pH 8.0, 20°C
Populus trichocarpa
17.4
-
2-(glutathione-S-yl)-menadione
wild-type, pH 8.0, 20°C
Populus trichocarpa
17.5
-
2-(glutathione-S-yl)-1,4-benzoquinone
wild-type, pH 8.0, 20°C
Populus trichocarpa
110.6
-
(glutathione-S-yl)-2-chloro-1,4-benzoquinone
wild-type, pH 8.0, 20°C
Populus trichocarpa
3215
-
2-(glutathione-S-yl)-menadiol
wild-type, pH 8.0, 20°C
Populus trichocarpa
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.95
-
S-glutathionylquercetin
wild-type, pH 8.0, 20°C
Populus trichocarpa
17.4
-
2-(glutathione-S-yl)-menadione
wild-type, pH 8.0, 20°C
Populus trichocarpa
17.5
-
2-(glutathione-S-yl)-1,4-benzoquinone
wild-type, pH 8.0, 20°C
Populus trichocarpa
110.6
-
(glutathione-S-yl)-2-chloro-1,4-benzoquinone
wild-type, pH 8.0, 20°C
Populus trichocarpa
3215
-
2-(glutathione-S-yl)-menadiol
wild-type, pH 8.0, 20°C
Populus trichocarpa
Other publictions for EC 1.8.5.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741262
Schwartz
Crystal structure of Saccharom ...
Saccharomyces cerevisiae
PLoS ONE
11
e0164678
2016
-
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1
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-
-
-
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1
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-
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1
-
1
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1
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1
-
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-
-
-
-
-
-
-
-
-
-
-
-
740452
Lallement
-
Glutathionyl-hydroquinone redu ...
Populus trichocarpa
FEBS Lett.
589
37-44
2015
-
-
-
1
2
-
-
5
1
-
-
-
-
1
-
-
-
-
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1
-
-
6
-
1
-
-
-
5
-
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1
2
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5
1
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1
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6
-
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5
-
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-
5
5
740055
Lam
-
Reduction of benzoquinones to ...
Cupriavidus necator, Cupriavidus necator JMP 134-1, Escherichia coli, Saccharomyces cerevisiae, Sphingobium chlorophenolicum, Sphingobium chlorophenolicum ATCC 29327
Biochemistry
51
5014-5021
2012
-
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20
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30
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30
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4
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20
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20
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30
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20
-
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20
20
740704
Green
Structural understanding of th ...
Escherichia coli
J. Biol. Chem.
287
35838-35848
2012
-
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1
4
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1
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1
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4
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1
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2
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1
4
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1
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4
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1
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-
740012
Xun
S-Glutathionyl-(chloro)hydroqu ...
Sphingobium chlorophenolicum
Biochem. J.
428
419-427
2010
-
-
1
-
-
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5
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2
2
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1
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7
1
1
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4
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1
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5
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2
2
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7
1
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4
-
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4
4
740614
Huang
Maintenance role of a glutathi ...
Sphingobium chlorophenolicum, Sphingobium chlorophenolicum ATCC 39723
J. Bacteriol.
190
7595-7600
2008
-
-
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4
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1
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6
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1
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1
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6
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1
1
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