BRENDA - Enzyme Database
show all sequences of 1.8.5.5

Sequence analysis of the phs operon in Salmonella typhimurium and the contribution of thiosulfate reduction to anaerobic energy metabolism

Heinzinger, N.K.; Fujimoto, S.Y.; Clark, M.A.; Moreno, M.S.; Barrett, E.L.; J. Bacteriol. 177, 2813-2820 (1995)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21300
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
28500
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
82700
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Organism
Organism
UniProt
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
P37600 and P0A1I1
P37600 i.e. subunit PhsA, P0A1I1 i.e. subunit PhsB
-
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
P37600 and P0A1I1
P37600 i.e. subunit PhsA, P0A1I1 i.e. subunit PhsB
-
Subunits
Subunits
Commentary
Organism
?
x * 82700, subunit PhsA, x * 21300, subunit PhsB, and x * 28500, subunit PhsC, calculated
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21300
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
28500
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
82700
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 82700, subunit PhsA, x * 21300, subunit PhsB, and x * 28500, subunit PhsC, calculated
Salmonella enterica subsp. enterica serovar Typhimurium
Expression
Organism
Commentary
Expression
Salmonella enterica subsp. enterica serovar Typhimurium
oxygen and thiosulfate control of Phs expression involves negative regulation
additional information
General Information
General Information
Commentary
Organism
physiological function
the Phs chromosomal locus of Salmonella typhimurium is essential for the dissimilatory anaerobic reduction of thiosulfate to hydrogen sulfide. The Phs region is a functional operon with three open reading frames, designated PhsA, PhsB, and PhsC, which encode peptides of 82.7, 21.3, and 28.5 kDa, respectively. The products of PhsA and PhsB exhibit significant homology with the catalytic and electron transfer subunits of anaerobic molybdoprotein oxidoreductases. The predicted PhsC product is highly hydrophobic and similar in size to the hydrophobic subunits of the molybdoprotein oxidoreductases containing subunits homologous to PhsA and PhsB. PhsABC appears to encode thiosulfate reductase
Salmonella enterica subsp. enterica serovar Typhimurium
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the Phs chromosomal locus of Salmonella typhimurium is essential for the dissimilatory anaerobic reduction of thiosulfate to hydrogen sulfide. The Phs region is a functional operon with three open reading frames, designated PhsA, PhsB, and PhsC, which encode peptides of 82.7, 21.3, and 28.5 kDa, respectively. The products of PhsA and PhsB exhibit significant homology with the catalytic and electron transfer subunits of anaerobic molybdoprotein oxidoreductases. The predicted PhsC product is highly hydrophobic and similar in size to the hydrophobic subunits of the molybdoprotein oxidoreductases containing subunits homologous to PhsA and PhsB. PhsABC appears to encode thiosulfate reductase
Salmonella enterica subsp. enterica serovar Typhimurium
Expression (protein specific)
Organism
Commentary
Expression
Salmonella enterica subsp. enterica serovar Typhimurium
oxygen and thiosulfate control of Phs expression involves negative regulation
additional information
Other publictions for EC 1.8.5.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725273
Stoffels
Thiosulfate reduction in Salmo ...
Salmonella enterica
J. Bacteriol.
194
475-485
2012
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1
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2
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1
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1
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2
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1
1
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645578
Bang
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Cadmium, lead, and zinc remova ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biotechnol. Lett.
22
1331-1335
2000
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1
1
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1
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1
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737488
Bang
Engineering hydrogen sulfide p ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
Appl. Environ. Microbiol.
66
3939-3944
2000
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1
1
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11
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1
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1
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738317
Alami
Cloning and characterization o ...
Salmonella enterica subsp. enterica serovar Typhimurium
Gene
156
53-57
1995
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8
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1
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1
1
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738502
Heinzinger
Sequence analysis of the phs o ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
J. Bacteriol.
177
2813-2820
1995
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3
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6
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1
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738491
Clark
The phs gene and hydrogen sulf ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
169
2391-2397
1987
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