Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.5.4 extracted from

  • Zhang, Y.; Weiner, J.
    Characterization of the kinetics and electron paramagnetic resonance spectroscopic properties of Acidithiobacillus ferrooxidans sulfide quinone oxidoreductase (SQR) (2014), Arch. Biochem. Biophys., 564, 110-119 .
    View publication on PubMed
Search for more literature for 1.8.5.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acidithiobacillus ferrooxidans

Protein Variants

Protein Variants Comment Organism
C128A the mutant shows strongly reduced activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
C128S the mutant shows strongly reduced activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
C160A the mutant shows severely reduced activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
C160S the mutant shows strongly reduced activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
C356A inactive Acidithiobacillus ferrooxidans
C356S the mutant shows severely reduced activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
H132A the mutant shows strongly reduced activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
H198A the mutant shows severely reduced activity compared to the wild type enzyme Acidithiobacillus ferrooxidans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00297
-
 Sulfide wild type enzyme, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
0.00343
-
 decylubiquinone wild type enzyme, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
0.00425
-
 decylubiquinone mutant enzyme C128A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
0.005
-
 Sulfide mutant enzyme H132A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
0.0054
-
 decylubiquinone mutant enzyme H132A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
0.00553
-
 Sulfide mutant enzyme C128S, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
0.0056
-
 Sulfide mutant enzyme C128A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
0.00585
-
 decylubiquinone mutant enzyme C128S, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans

Organism

Organism UniProt Comment Textmining
Acidithiobacillus ferrooxidans B7JBP8
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfide + decylubiquinone
-
 Acidithiobacillus ferrooxidans sulfur + decylubiquinol
-
 ?

Subunits

Subunits Comment Organism
? x * 49000, SDS-PAGE Acidithiobacillus ferrooxidans

Synonyms

Synonyms Comment Organism
SQR
-
 Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Acidithiobacillus ferrooxidans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 45 the recombinant enzyme is highly stable when incubated at 0°C, 4°C, 23°C or 30°C with more than 90% of the activity remaining after 7 h. It is less stable at 37°C and loses about 40% of the activity in the first 7.5 h. The activity of the enzyme decreases more rapidly at 45°C with a half-life of approximately 5 h Acidithiobacillus ferrooxidans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 Sulfide mutant enzyme C160A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
0.1
-
 Sulfide mutant enzyme C356S, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
0.3
-
 Sulfide mutant enzyme C198A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
0.5
-
 Sulfide mutant enzyme C160S, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1
-
 Sulfide mutant enzyme C128A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.4
-
 Sulfide mutant enzyme H132A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.6
-
 Sulfide mutant enzyme C128S, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
6.5
-
 Sulfide wild type enzyme, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Acidithiobacillus ferrooxidans

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Acidithiobacillus ferrooxidans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
180
-
 Sulfide mutant enzyme C128A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
240
-
 decylubiquinone mutant enzyme C128A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
250
-
 decylubiquinone mutant enzyme H132A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
270
-
 decylubiquinone mutant enzyme C128S, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
270
-
 Sulfide mutant enzyme H132A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
290
-
 Sulfide mutant enzyme C128S, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1890
-
 decylubiquinone wild type enzyme, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
2190
-
 Sulfide wild type enzyme, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans